HPA002689
Anti-WASHC1 antibody produced in rabbit
Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonyme(s) :
Anti-FAM39E, Anti-FLJ00038, Anti-WASH1
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
A propos de cet article
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC
Citations:
14
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderbiological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
product line
Prestige Antibodies® Powered by Atlas Antibodies
form
buffered aqueous glycerol solution
species reactivity
human
enhanced validation
RNAi knockdown
Learn more about Antibody Enhanced Validation
technique(s)
immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:1000-1:2500
immunogen sequence
GAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKKKQKEQEQVRATSQGGHLMSDLFNKLVMRHKGISGKGPGAGEGPGGAFARVSDSIPPLPPPQQPQVDEDEDDWES
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... WASH1(100287171)
General description
WASH complex subunit 1 (WASHC1) is located on human chromosome 9p24.3. It belongs to Wiskott-Aldrich Syndrome Protein and SCAR Homolog (WASH) family. WASHC1 protein has an actin binding domain.
Immunogen
WASH complex subunit 1 recombinant protein epitope signature tag (PrEST)
Application
Anti-WASHC1 antibody produced in rabbit has been used:
- in the detection of WASH complex in Dictyostelium cells by immunofluorescence imaging.
- in the detection of WASH proteins in HeLa cells using confocal microscopy.
- in western blotting for the detection of WASH1 complexes in human leukemic Jurkat T cells.
Biochem/physiol Actions
WASH exists as multiple complex protein in HeLa and bovine cells. WASH proteins coordinate the migration of endosomal α5β1 integrin. WASHC1 protein regulates actin cytoskeleton organisation. WASH1 protein is regarded as actin-nucleating promoting factor. WASH protein complex interacts with actin related protein, Arp2/3 and mediates actin networking.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
Other Notes
Corresponding Antigen APREST74318
Legal Information
Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
Still not finding the right product?
Essayez notre Outil de sélection de produits pour affiner vos choix.
Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors
Buckley CM, et al.
Proceedings of the National Academy of Sciences of the USA, 113(40), E5906-E5915 (2016)
The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex
Derivery E, et al.
Developmental Cell, 17(5), 712-723 (2009)
Catherine M Buckley et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(40), E5906-E5915 (2016-09-21)
Macropinocytosis is an ancient mechanism that allows cells to harvest nutrients from extracellular media, which also allows immune cells to sample antigens from their surroundings. During macropinosome formation, bulk plasma membrane is internalized with all its integral proteins. It is