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Merck

I1146

R3 IGF-1 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonyme(s) :

Human IGF-1, IGF-1 Protein, Recombinant IGF-1

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A propos de cet article

NACRES:
NA.77
UNSPSC Code:
51111800
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥85% (HPLC)
Form:
lyophilized powder
Mol wt:
7,676 Da
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biological source

human

Quality Level

recombinant

expressed in E. coli

assay

≥85% (HPLC)

form

lyophilized powder

potency

≤10 ng/mL ED50

quality

endotoxin tested

mol wt

7,676 Da

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

2-8°C

Application

R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.

Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.

Biochem/physiol Actions

Proliferation of chinese hamster ovary CHO cells.

Preparation Note

Prepared from 0.1 M acetic acid

Other Notes

Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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We previously showed that the orphan nuclear receptor Nur77 (Nr4a1) plays an important role in the regulation of glucose homeostasis and oxidative metabolism in skeletal muscle. Here, we show using both gain- and loss-of-function models that Nur77 is also a

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