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About This Item
Nom du produit
IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder
conjugate
unconjugated
grade
reagent grade
assay
≥95% (SDS-PAGE)
form
lyophilized powder
composition
Protein, ≥80%
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
Purified mouse IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA and chromatin immunoprecipitation assay. It was used to coat nano particles in the development of biosensors.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Mouse IgG is purified from normal mouse serum by fractionation and ion-exchange chromatography.
Mouse IgG is purified from normal mouse serum by fractionation and ion-exchange chromatography.
Physical form
Lyophilized from 0.01 M phosphate buffer, pH 7.2, with 0.015 M NaCl
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Lonnie J Lucas et al.
Biosensors & bioelectronics, 23(5), 675-681 (2007-09-18)
Double detection of microsphere light scattering and quantum dot emission was demonstrated for lab-on-a-chip immunoassay without using stationary support. We conjugated quantum dots (QDs) onto microspheres to enable multiplex assays as well as to enhance the limit of detection (LOD).
Fluorescent Biorecognition of Gold Nanoparticle-IgG Conjugates Self-Assembled on E-Beam Patterns
Powell T and Yoon JY
Biotechnol. Progress, 22, 106-110 (2006)
Panojot Bifsha et al.
Molecular neurobiology, 54(7), 4921-4935 (2016-08-16)
Dysfunction of midbrain dopaminergic (mDA) neurons is involved in Parkinson's disease (PD) and neuropsychiatric disorders. Pitx3 is expressed in mDA neuron subsets of the substantia nigra compacta (SNc) and of the ventral tegmental area (VTA) that are degeneration-sensitive in PD.
Paurvi Shinde et al.
Journal of leukocyte biology, 102(1), 57-69 (2017-04-23)
LPS is a powerful adjuvant, and although LPS-mediated TLR4 signaling has been exquisitely delineated, the in vivo mechanism of how TLR4 responses impact T cell priming is far less clear. Besides costimulation, TNF and type 1 IFN are dominant cytokines
C Rebouissou et al.
Blood, 91(12), 4727-4737 (1998-06-17)
Agonist antihuman gp130 transducer monoclonal antibodies (MoAbs) were used in SCID mice to grow myeloma cells whose survival and proliferation is dependent on gp130 transducer activation. The agonist anti-gp130 MoAbs neither bound to murine gp130 nor activated murine cells and
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