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Merck

M5017

Medium 199

With Earle′s salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199

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A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.71
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Nom du produit

Medium 199, With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
phenol red: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

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General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

Classe de stockage

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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