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Merck

M5519

Milieu de base de Murashige et Skoog

powder, suitable for plant cell culture

Synonyme(s) :

Milieu de base MS

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A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.72
MDL number:
MFCD00240976

Principaux documents

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form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

Quality Level

200

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Catégories apparentées

General description

Le milieu de Murashige et Skoog est un milieu de culture des tissus de plantes largement utilisé. Le milieu de base M&S contient des macronutriments qui incluent des taux élevés de nitrates et des additifs organiques comme de la gélose, des sucres, des vitamines et des régulateurs de croissance. Parmi les régulateurs de croissance importants ajoutés au milieu M&S, on compte l′auxine/morphogène IAA et la kinétine, une cytokinine et agent promoteur de la division cellulaire.

Application

Le milieu de Murashige et Skoog a été utilisé dans le milieu de culture des tissus de plantes pour l′étude de la transformation de plantes.

Preparation Note

Comporte des macronutriments, des micronutriments et des vitamines comme décrit par Murashige et Skoog (1962).

Formulation du milieu
Le milieu de Murashige et Skoog peut être reconstitué à partir d′une poudre ou en combinant des produits constituants majeurs du milieu complet M&S, dont les mélanges de macronutriments et de vitamines. Le milieu de Murashige et Skoog (M5519) contient les micronutriments et vitamines de la formulation classique d′origine. Il peut être additionné de saccharose, de gélose, d′auxines (IAA) et de cytokinines (kinétine) pour former un milieu complet apte à la croissance des tissus de plantes.
Produit formulé pour contenir 4,4 grammes de poudre par litre de milieu.

pictograms

Flame over circleExclamation mark
GHS03,GHS07

signalword

Warning

hcodes

H272,H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Classe de stockage

5.1B - Oxidizing hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000457359
0000457359
0000423246
0000423246
0000422941

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the formulation of Product M5519, Murashige and Skoog (MS) Basal Medium?

    It is media containing the macro- and micronutrients, and vitamins.

  4. What is the difference between Product M5519, Murashige and Skoog (MS) Basal Medium and M5524, Murashige and Skoog Basal Salt Mixture?

    Product M5519 is the complete medium whereas M5524 is just the basal salt medium.

  5. Product M5519, Murashige and Skoog Basal Medium, is on back order. Can you suggest an alternate?

    When M5519 is on backorder, you can purchase Murashige and Skoog Basal Salt Mixture (Product No. M5524) and Murashige and Skoog Vitamin Powder (Product No. M7150). You will use 4.3 g/L of M5524 (rather than 4.4 g/L of M5519) and 1 mL/L of M7150. When combined, the salt and organic content is the same as Product No. M5519.

  6. How much agar is there in Product M5519, Murashige and Skoog Basal Medium?

    This product does not contain any agar.

  7. Product M5519, Murashige and Skoog Basal Medium, has precipitates; will that affect the growth?

    Precipitates are known to occur, with time, in plant tissue culture media.  The precipitates have been analyzed.  They are composed of small pale yellow-white particles.  Analysis of precipitates indicated a predominance of iron, phosphate, and zinc.  The probable cause of the precipitates is the inevitable oxidation of ferrous ions to ferric ions and the presence of unchelated ferric ions.  When the solubility of ferric phosphate is exceeded precipitation occurs.  There are no reports of detrimental effects on growth and development in plant tissue culture due to the precipitates.

  8. Can I autoclave Product M5519, Murashige and Skoog Basal Medium?

    We would not suggest autoclaving the media, because a precipitate may form.  It is advisable to add pre-autoclaved water as it contains less oxygen; this reduces the likelihood of oxidation of ferrous to ferric ions.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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