M5774
Anti-Mouse Serum antibody produced in rabbit
whole antiserum
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A propos de cet article
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IEP
Citations:
22
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Laissez-nous vous aiderbiological source
rabbit
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
contains
15 mM sodium azide
species reactivity
mouse
technique(s)
immunoelectrophoresis: suitable
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Mouse serum comprises hormones, antigens, electrolytes and antibodies. It serves blocking agent in immunoassays.
Application
Anti-Mouse Serum antibody produced in rabbit has been used in differential fluorescent labelling. It has also been used in differential staining of inner cell mass (ICM) and and trophectoderm (TE) cells. It has been also been used in the removal of zona pellucidae for the isolation of inner cell mass (ICM) from mouse blastocysts.
Anti-Mouse Serum antibody produced in rabbit was used as blocking agent in differential cell count of trophectoderm and inner cell mass cells of mouse embryos.
Biochem/physiol Actions
Strong reactivity with normal mouse serum has been determined by immunoelectrophoresis (IEP). This antiserum has not been assayed for interspecies crossreactivity.
Physical form
Rabbit Anti-Mouse Serum is provided as a liquid containing 15 mM sodium azide as preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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J Van der Elst et al.
Human reproduction (Oxford, England), 13(6), 1595-1599 (1998-08-04)
We demonstrated previously that ultra-rapid freezing of mouse oocytes with 3.5 M dimethylsulphoxide (DMSO) decreased cell numbers in day 5 in-vitro cultured blastocysts. In the present study we counted cell numbers of trophectoderm (TE) and inner cell mass (ICM) separately
Namfon Inna et al.
Clinical and experimental reproductive medicine, 45(3), 110-115 (2018-09-12)
To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts. Two-cell mouse embryos were randomly allocated into three groups: a non-frozen control group (group
Both the folate cycle and betaine-homocysteine methyltransferase contribute methyl groups for DNA methylation in mouse blastocysts
Zhang B, et al.
Faseb Journal, 29(3), 1069-1079 (2015)
Raúl Fernández-González et al.
Reproduction (Cambridge, England), 137(2), 271-283 (2008-11-20)
We have reported that in vitro culture (IVC) of preimplantation mouse embryos in the presence of FCS produces long-term effects (LTE) on development, growth and behaviour of the offspring at adult age. To analyse the mechanisms underlying this phenomenon, we
Carla Mulas et al.
Development (Cambridge, England), 146(6) (2019-03-28)
The power of mouse embryonic stem (ES) cells to colonise the developing embryo has revolutionised mammalian developmental genetics and stem cell research. This power is vulnerable, however, to the cell culture environment, deficiencies in which can lead to cellular heterogeneity
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