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Merck

M6395

MCDB 105 Medium

With trace elements, L-glutamine and 25mM HEPES, powder, suitable for cell culture

Synonyme(s) :

Molecular, Cellular, and Development Biology 105 medium

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A propos de cet article

NACRES:
NA.75
UNSPSC Code:
12352207
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Nom du produit

MCDB 105 Medium, With trace elements, L-glutamine and 25mM HEPES, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.001242 g/L
glucose: 0.72064 g/L (Dextro)
sodium pyruvate: 0.11 g/L
HEPES: 5.958 g/L (25mM)
L-glutamine: 0.3653 g/L

shipped in

ambient

storage temp.

2-8°C

Quality Level

Application

MCDB 105 Medium has been used to culture:
  • human umbilical vein endothelial cells
  • primary human endometrial stromal cells (ESC)
  • ovarian cancer cell line (OVCAR3)

General description

MCDB media has been designed for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). Each MCDB medium is formulated (qualitatively and quantitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes the growth of a specific cell type. MCDB 105 is derived from the modifications done with MCDB 104 medium. This medium is suitable for the long-term growth of WI-38, MRC-5, and other human diploid fibroblast-like cells.

Preparation Note

Formulated to contain 14.9 grams of powder per liter of medium.

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Bjørn Grinde et al.
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Jing-Jing Wang et al.
Oncoimmunology, 8(11), e1659092-e1659092 (2019-10-28)
Blockade of the programmed cell death 1(PD-1)/PD-1 ligand-1(PD-L1) pathway has been exploited therapeutically in many cancer types. Upregulation of PD-L1 in tumor cells contributes to malignancy through suppression of the T cell-mediated antitumor response. Pyruvate dehydrogenase kinase 1 (PDK1), a
Minghai Shao et al.
Nucleic acids research, 39(10), 4035-4047 (2011-01-27)
Platelet derived growth factor (PDGF) regulates gene transcription by binding to specific receptors. PDGF plays a critical role in oncogenesis in brain and other tumors, regulates angiogenesis, and remodels the stroma in physiologic conditions. Here, we show by using microRNA
Jana Kim et al.
Journal of magnetic resonance imaging : JMRI, 47(6), 1589-1600 (2017-12-06)
Steady state susceptibility contrast (SSC)-MRI provides information on vascular morphology but is a rarely used method. To investigate the utility of the ultrasmall superparamagnetic iron oxide particles (USPIOs) GEH121333 for measuring tumor response to bevacizumab and compare this with gadolinium-based
Tongcui Ma et al.
eLife, 9 (2020-05-27)
The female reproductive tract (FRT) is the most common site of infection during HIV transmission to women, but viral remodeling complicates characterization of cells targeted for infection. Here, we report extensive phenotypic analyses of HIV-infected endometrial cells by CyTOF, and

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