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Merck

M9004

Malic Dehydrogenase from bovine heart

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

Synonyme(s) :

L-Malate:NAD+ oxidoreductase, MDH

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A propos de cet article

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-622-5
MDL number:
Numéro CE :
Specific activity:
2000-4000 units/mg protein (modified Warburg-Christian)
Biological source:
bovine heart
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biological source

bovine heart

form

ammonium sulfate suspension

specific activity

2000-4000 units/mg protein (modified Warburg-Christian)

mol wt

35 kDa

impurities

≤0.01% Glutamic-Oxalacetic Transaminase, ≤0.01% Glutamic-Pyruvic Transaminase

storage temp.

2-8°C

Quality Level

General description

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Application

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

Physical form

Suspension in 3 M (NH4)2SO4 - 0.01 M KH2PO4 solution, pH 7.3

Other Notes

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Z Y Chen et al.
Plant physiology, 112(2), 677-684 (1996-10-01)
At low-CO2 (air) conditions, the unicellular green alga Chlamydomonas reinhardtii acquires the ability to raise its internal inorganic carbon concentration. To study this adaptation to low CO2, cDNA clones induced under low-CO2 growth conditions were selected through differential screening. One
Isoelectric points and molecular weights of proteins: a new table.
Righetti, P.G., and Tudor, G.
Journal of Chromatography A, 220, 115-194 (1981)
J D Eisenback et al.
Journal of nematology, 35(4), 395-403 (2003-12-01)
Meloidogyne haplanaria n. sp. is described and illustrated from specimens parasitizing peanut in Texas. The perineal pattern of the female is rounded to oval with a dorsal arch that is high and rounded except for striae near the vulva, which
Eisaku Hokazono et al.
Clinica chimica acta; international journal of clinical chemistry, 412(15-16), 1436-1440 (2011-05-10)
In human serum, as for phospholipids not containing choline, phosphatidylethanolamine (PE) exists approximately 5% in a whole phospholipid. PE is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis
Flow injection system for on-line monitoring of fumaric acid in biological processes
Rhee, J.
Analytica Chimica Acta, 499, 71-80 (2003)

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