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Merck

M9309

McCoy′s 5A Medium

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

5A basal medium

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A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
MFCD00217560

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Nom du produit

McCoy′s 5A Medium, Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

Quality Level

300

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Catégories apparentées

Application

McCoy′s 5A Medium has been used:
  • to culture HCT-116?cells
  • to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
  • to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

General description

The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000411517
0000411517
0000361280
0000361280
0000336157

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