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Merck

MAK094

Protein Carbonyl Content Assay Kit

sufficient for 100 colorimetric tests

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A propos de cet article

NACRES:
NA.84
UNSPSC Code:
12161503
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Nom du produit

Protein Carbonyl Content Assay Kit, sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cardiovascular diseases; neurological disorders; cancer; gastrointestinal diseases

storage temp.

2-8°C

Application

Protein carbonyl content assay kit has been used to determine protein carbonyl concentration.
Suitable for quantifying carbonyls in protein samples

General description

Oxidative stress results when the effectiveness of antioxidant defenses is insufficient to deal with the production of reactive oxygen species (ROS). ROS can induce damage to DNA, lipids, and proteins. The oxidation of proteins results in the production of stable carbonyl groups, which can be used as a measure of oxidative injury.

Biochem/physiol Actions

The Protein Carbonyl Content Assay Kit provides a simple and direct procedure for measuring carbonyl content in a variety of biological samples. Carbonyl content is determined by the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) leading to the formation of stable dinitrophenyl (DNP) hydrazone adducts, which can be detected spectrophotometrically at 375 nm, proportional to the carbonyls present.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1 - STOT SE 3

target_organs

Respiratory system

Classe de stockage

8A - Combustible corrosive hazardous materials


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Mohammad Arif et al.
Toxicology and industrial health, 34(4), 213-218 (2018-03-10)
Lead exposure can damage cells directly by effecting DNA or indirectly by modifying proteins and enzymes. In Bangladesh, many working children are exposed to a very high level of lead during their early life due to their involvement with lead-oriented
J O Zeitz et al.
Journal of animal physiology and animal nutrition, 102(4), 999-1011 (2018-05-01)
This study hypothesized that plasma and tissue antioxidant status of broilers is positively influenced when dietary Met concentrations exceed, and negatively when they go below NRC recommendations. In addition, different Met sources are hypothesized to affect the antioxidant defence system
Melissa M Miranda-Cruz et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 213, 19-26 (2018-07-25)
Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes involved in glucose metabolism. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces a shift in glucose metabolism
Effects of chilled-then-frozen storage (up to 52 weeks) on an indicator of protein oxidation and indices of protein degradation in lamb M. longissimus lumborum.
Coombs CE, et al.
Meat Science, 135, 134-141 (2018)
Yujie Huang et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 49(2), 758-779 (2018-08-31)
Skeletal muscle atrophy is an important health issue and can impose tremendous economic burdens on healthcare systems. Glucocorticoids (GCs) are well-known factors that result in muscle atrophy observed in numerous pathological conditions. Therefore, the development of effective and safe therapeutic

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