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P4557

Phénol solution

Name: Phénol solution
Brand: SIGMA

BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, Molecular Biology

Synonyme(s) :

Tris-equilabrated Phenol

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A propos de cet article

Formule empirique (notation de Hill) :

C₆H₆O

Numéro CAS:

108-95-2

Poids moléculaire :

94.11

UNSPSC Code:

12352104

NACRES:

NA.52

PubChem Substance ID:

57654529

EC Number:

203-632-7

Beilstein/REAXYS Number:

6428463

MDL number:

MFCD00002143

Assay:

≥99.0% (GC)

Grade:

Molecular Biology

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Propriétés

grade

Molecular Biology

Quality Level

200

product line

BioReagent

assay

≥99.0% (GC)

form

liquid

dilution

(for analytical testing)

pH

7.7-8.1 (25 °C)

storage temp.

2-8°C

SMILES string

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Gene Information

human ... GABRA1(2554)

Description

General description

A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
for extraction of DNA from Arabidopsis thaliana Rosette leaves
in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
as a component of extraction buffer to extract RNA from fruit pulp
for the extraction of protein from roots
for the isolation of fecal DNA
for the extraction of caged RNA

Biochem/physiol Actions

The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.