P5306
Papaïne from papaya latex
lyophilized powder, aseptically filled
Synonyme(s) :
Papaïnase
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-627-2
MDL number:
Specific activity:
≥8.0 units/mg protein
Biological source:
papaya (latex)
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Laissez-nous vous aiderbiological source
papaya (latex)
sterility
aseptically filled
form
lyophilized powder
specific activity
≥8.0 units/mg protein
mol wt
23.4 kDa
solubility
H2O: soluble 1.2 mg/mL
storage temp.
−20°C
Quality Level
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Catégories apparentées
General description
Papain is a thermostable enzyme and becomes inactive at 55°C. It has two domains and the catalytic region is present at the domain interface. The cysteine 25 in the active site performs the proteolysis by nucleophilic attack on the carbonyl carbon of a peptides. Papain has wide industrial applications in food and pharmaceutical industry.
Application
Sert à produire des fragments Fab d′anticorps. Également utilisée pour la dissociation cellulaire, car des études ont montré qu′elle s′avère plus efficace et moins détériorante avec certains tissus.
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies . It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P5306 is provided as a lyophilized powder that is aseptically filled. It has been used to digest mouse retinas to study the RAGE axis in nonproliferative diabetic retinopathy (NPDR).
The product from Sigma has been used in the digestion of extracellular matrix (ECM). It has also been used with other proteolytic enzymes for in situ protein degradation to produce crystals suitable for structure determination.
Biochem/physiol Actions
Protéase à cystéine qui clive les liaisons peptidiques des acides aminés basiques, de la leucine ou de la glycine.
pH optimal situé entre 6,0 et 7,0.
Hydrolyse également les esters et les amides.
pH optimal situé entre 6,0 et 7,0.
Hydrolyse également les esters et les amides.
Papain papaya latex has antifungal activity against C. albicans. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Preparation Note
Prepared from P 4762
Other Notes
Pour en savoir plus sur la papaïne, rendez-vous sur www.sigma-aldrich.com/enzymeexplorer.
One unit will hydrolyze 1.0 μmole of BAEE per minute at pH 6.2 at 25 deg C.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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