P5994
Substrat de phosphatase
40 mg tablets
Synonyme(s) :
Phosphate de 4-nitrophényle disodium salt hexahydrate, pNPP disodium salt hexahydrate
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A propos de cet article
Formule linéaire :
O2NC6H4OP(O)(ONa)2 · 6H2O
Numéro CAS:
Poids moléculaire :
371.14
NACRES:
NA.54
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
Service technique
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form
tablet
concentration
36-44 mg/tablet
impurities
p-nitrophenol, essentially free
solubility
H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)
storage temp.
−20°C
SMILES string
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
General description
Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.
Application
Substrat chromogène utilisé dans les procédures ELISA avec phosphatase alcaline et acide. Génère un produit final soluble de couleur jaune qui peut être mesuré par spectrophotométrie à 405 nm. La réaction du pNPP peut être stoppée à l′aide de NaOH 3 N puis mesurée à 405 nm.
Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.
Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).
Preparation Note
Contains 40 mg substrate per tablet
Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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