P6611
Gel d′affinité au nickel HIS-Select®
(1:1 suspension in a 20% ethanol solution)
Synonyme(s) :
Ni-NTA resin, nickel charged agarose
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A propos de cet article
conjugate
magnetic beads
form
(1:1 suspension in a 20% ethanol solution)
feature
hydrophilic
packaging
pkg of 1 mL, pkg of 100 mL, pkg of 25 mL, pkg of 5 mL, pkg of 500 mL
concentration
1.5-2.4 mL/mL (suspension in packed gel)
technique(s)
protein purification: suitable
color
faint blue to very dark blue
matrix
6% Beaded Agarose
capacity
>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
transition temp
flash point 32 °C (closed cup)
storage temp.
2-8°C
Quality Level
General description
Application
Features and Benefits
- Grande sélectivité, pour une plus grande pureté
- Liaison hydrophile non chargée inédite qui réduit l'adhésion non spécifique
- Capacité de fixation supérieure à 15 mg/ml pour les protéines marquées à l'histidine
- Fixation en conditions dénaturantes ou non dénaturantes
- Purification en une seule étape
- Interactions ioniques indésirables réduites au minimum
- Très faible élution du nickel
- Taille des billes : 45 à 165 μm
Physical form
Preparation Note
Other Notes
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Classe de stockage
3 - Flammable liquids
wgk
WGK 3
flash_point_f
89.6 °F - closed cup
flash_point_c
32 °C - closed cup
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Can imidazole be used with HIS-Select® Nickel Affinity Gel, Product P6611?
For column chromatography, no more than 20 mM is suggested in the extract, equilibration, and wash buffers to prevent non-specific binding of proteins. No more than 250 mM is suggested for the elution buffers. Many proteins will elute with imidazole levels as low as 100 to 200 mM. For batch methods the imidazole concentration may have to be reduced or eliminated.When a protein is expressed at low levels, the presence of the imidazole limits the binding of the protein in the batch method but not when used in a column.
Can Tris buffers be used instead of phosphate buffer for HIS-Select® Nickel Affinity Gel, Product P6611?
Yes, Tris buffers should work.
Why won't my recombinant protein with a histidine-containing tag bind to HIS-Select® Nickel Affinity Gel, Product P6611?
Verify the pH and composition of sample and equilibration buffers. Make sure there are no chelating or reducing agents present in the extraction buffer. If using the batch mode, remove imidazole. Run the affinity purification under denaturing conditions. Run a Western blot of the extract to verify that the recombinant protein is present.
Can I use SDS with HIS-Select® Nickel Affinity Gel, Product P6611?
0.1% SDS has been used with some samples, with no adverse effects on the observed protein binding. However, SDS will effectively coat proteins and may block the binding to the column. It is probably very protein-specific and an SDS concentration that works for one protein may not work for another.
What needs to be done if the HIS-Select® Nickel Affinity Gel, Product P6611, resin turns brown on reuse?
During purification many protein extracts tend to discolor an affinity gel during the loading step. The original color will return after the wash or elution step. If the color is still not changing strip and recharge the affinity gel with nickel. Wash with EDTA and recharge with Nickel solution.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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