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Merck

P7002

Phospho(enol)pyruvic acid trisodium salt hydrate

≥97% (enzymatic), powder

Synonyme(s) :

2-(Phosphonooxy)-2-propenoic acid trisodium salt, PEP

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A propos de cet article

Formule linéaire :
C3H2O6PNa3 · x H2O
Numéro CAS:
5541-93-5
Poids moléculaire :
233.99 (anhydrous basis)
UNSPSC Code:
12352204
PubChem Substance ID:
24898810
NACRES:
NA.32
MDL number:
MFCD00150734

Principaux documents

Voir toute la documentation
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Nom du produit

Phospho(enol)pyruvic acid trisodium salt hydrate, ≥97% (enzymatic)

SMILES string

[Na].OC(=O)C(=C)OP(O)(O)=O

InChI

1S/C3H5O6P.Na.H/c1-2(3(4)5)9-10(6,7)8;;/h1H2,(H,4,5)(H2,6,7,8);;

InChI key

KZPUEZVJSLLABH-UHFFFAOYSA-N

assay

≥97% (enzymatic)

form

powder

solubility

water: 100 mg/mL, clear to slightly hazy, colorless to very faintly yellow

storage temp.

−20°C

Quality Level

200

Application

Phospho(enol)pyruvic acid trisodium salt hydrate has been used in:
  • several enzyme activity assays, such as PRPP synthetase (PRPPS) assay, ribokinase (RK) assay, adenine phosphoribosyltransferase (APRT) assay
  • in the reaction mixture for enzyme kinetic assay to determine platelet-derived growth factor receptor α (PDGFRA) kinetic parameters
  • as a component in buffer B to react with adenosine diphosphate (ADP) to form adenosine triphosphate (ATP) for energy charge measurement

Biochem/physiol Actions

Phospho(enol)pyruvic acid (PEP) is involved in glycolysis and gluconeogenesis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.
Phosphoenolpyruvic acid plays a major role in the initial step of glycolysis by acting as an inhibitor of hexokinase, phosphoglucose isomerase, phosphofructokinase and aldolase.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
BCCN4406
BCCM8997
BCCL7776
BCCK8186
BCCG7369

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Consulter la Bibliothèque de documents

Deuterated nucleotides as chemical probes of RNA structure: a detailed protocol for the enzymatic synthesis of a complete set of nucleotides specifically deuterated at ribose carbons
Azad R, et al.
ScienceOpen Research (2015)
Kento Tokuyama et al.
Biotechnology and bioengineering, 115(6), 1542-1551 (2018-02-20)
Gene deletion strategies using flux balance analysis (FBA) have improved the growth-coupled production of various compounds. However, the productivities were often below the expectation because the cells failed to adapt to these genetic perturbations. Here, we demonstrate the productivity of
Structural and biochemical studies of the PDGFRA kinase domain
Liang L, et al.
Biochemical and Biophysical Research Communications, 477(4), 667-672 (2016)
Casimir Ledoux Sofeu et al.
PLoS neglected tropical diseases, 12(9), e0006597-e0006597 (2018-09-07)
Canine rabies is endemic in Cameroon, but human rabies exposures and cases are likely underreported because of inadequate surveillance. In 2014, the surveillance network in the West region of Cameroon was reinforced by introducing a new anti-rabies center, a framework
Sonja Schmid et al.
The Journal of chemical physics, 148(12), 123312-123312 (2018-04-02)
Single molecule time traces reveal the time evolution of unsynchronized kinetic systems. Especially single molecule Förster resonance energy transfer (smFRET) provides access to enzymatically important time scales, combined with molecular distance resolution and minimal interference with the sample. Yet the
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