PKH67GL
Kit de lieur cellulaire à fluorescence verte au PKH67 pour le marquage des membranes cellulaires à usage général
Distributed for Phanos Technologies
Synonyme(s) :
Green PKH membrane labeling kit
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A propos de cet article
packaging
pkg of 1 kit
storage condition
protect from light
fluorescence
λex 490 nm; λem 502 nm (PKH67 dye)
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
Quality Level
Application
Une perte lente de fluorescence a été observée dans des études in vivo utilisant le PKH1 et le PKH2. Le PKH67 peut présenter des propriétés similaires puisque ce comportement semble être caractéristique des colorants de liaison des cellules vertes, mais pas des colorants de liaison des cellules rouges. La corrélation entre la rétention in vitro de la membrane cellulaire et la demi-vie de fluorescence in vivo dans des cellules non en division permet de prévoir une demi-vie de fluorescence in vivo de 10-12 jours pour la PKH67. D'autres colorants verts de liaison cellulaire ayant une demi-vie similaire ont été utilisés pour suivre le trafic des lymphocytes et des macrophages in vivo sur des périodes de 1 à 2 mois, ce qui suggère que le PKH67 sera également utile pour des études de suivi in vivo de durée moyenne.
- marquage et analyse des exosomes expulsés par les cellules dans le cancer du sein triple négatif
- marquage des cellules apoptotiques afin d'étudier le rôle joué par le récepteur de l'ανβ5 dans la liaison et l'internalisation des cellules apoptotiques
- imagerie en fluorescence
Other Notes
Legal Information
Composants de kit seuls
- Diluent C 6 x 10
- PKH67 Cell Linker in ethanol .5 mL
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Classe de stockage
3 - Flammable liquids
flash_point_f
57.2 °F - closed cup
flash_point_c
14 °C - closed cup
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Does the dye leak from the cells after labeling when using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling?
In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.
When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?
Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.
Are cells lost during the PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling staining process?
Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery. Methods for improving cell viability can be found on the troubleshooting guide.
What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?
A protocol for visualization of tissue sections can be found in the technical bulletin. For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells. If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the difference between Green Fluorescent Cell Linker Kits PKH2 and PKH67?
PKH2 was one of the early PKH dyes. The PKH67 dye has a longer aliphatic tail. There is reduced cell-celldye transfer for PKH67 as compared with PKH2.
How many cells can be stained with Product PKH67GL, Green Fluorescent Cell Linker Kit?
This kit can stain 50 × 107 cells if used as directed (1 × 107 cells stained with 2 × 10-6 M PKH67).
What is the excitation and emission spectra for Product PKH67GL, Green Fluorescent Cell Linker Kit?
The spectra can be found on the product insert.The product has a maximum excitation at 490 nm and maximum emission at 502 nm.
Will Product PKH67, Green Fluorescent Cell Linker Kit, stain dead cells?
As long as the cell has an intact membrane, the PKH76 dye can label the cell.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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