R1280
Monoclonal Anti-RPA/p34 antibody produced in mouse
~2 mg/mL, clone 9H8, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
Anti-REPA2, Anti-RPA2, Anti-RPA32, Anti-Replication protein A2; 32kDa
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A propos de cet article
Conjugate:
unconjugated
Clone:
9H8, monoclonal
Application:
ICC, IP, WB
Citations:
4
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Laissez-nous vous aiderbiological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9H8, monoclonal
form
buffered aqueous solution
mol wt
antigen ~34 kDa
species reactivity
human, monkey
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.1-0.2 μg/mL using HeLa total cell extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RPA2(6118)
General description
Monoclonal Anti-RPA/p34 (mouse IgG1 isotype) is derived from the hybridoma 9H8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human RPA/p34. Replication protein A Replication protein A (RPA) complex is a single-stranded DNA-binding protein and it is made up of 70-, 32- and 14-kDa subunits.
Immunogen
purified human RPA/p34.
Application
The antibody may be used in:
- immunoblotting (~34 kDa)
- immunocytochemistry
- immunoprecipitation
Biochem/physiol Actions
Replication protein A (RPA) complex is essential for processes like DNA replication, recombination, and DNA repair pathways. The 32-kDa subunit of human RPA is hyper-phosphorylated in its N-terminus upon cellular DNA damage. This phosphorylation causes a change in RPA conformation that down-regulates its activity in DNA replication but does not affect DNA repair processes. Thus the role of RPA phosphorylation in the cellular response to DNA damage is to help regulate DNA metabolism and promote DNA repair.The RPA complex, once bound to single stranded DNA, recruits the ATR (ataxia-telangiectasia mutated and Rad3-related)-ATRIP (ATR-interacting protein) protein kinase complex that is crucial for the cellular response to replication stress and DNA damage and for ATR-mediated Chk1 activation in human cells. Furthermore, the association of this complex to the single stranded DNA causes the phosphorylation of RAD17 protein that is bound to DNA. Yeast strains deficient in RPA are defective in recruiting Ddc2 (yeast homologue of ATRIP) and thus down stream proteins are not phosphorylated. Replication protein A binds with the ssDNA with great affinity and interacts specifically with multiple proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
Zou L and Elledge S J
Science (New York, N.Y.), 300(5625), 1542-1548 (2003)
W Q Qiu et al.
The Journal of biological chemistry, 271(14), 8443-8451 (1996-04-05)
Progressive cerebral deposition of the amyloid beta-peptide (Abeta) is an early and constant feature of Alzheimer's disease. Abeta is derived by proteolysis from the beta-amyloid precursor protein. beta-Amyloid precursor protein processing and the generation of Abeta have been extensively characterized
Replication protein A phosphorylation and the cellular response to DNA damage
Binz S K, et al.
DNA Repair, 3(8-9), 1015-1024 (2004)
M K Kenny et al.
The Journal of biological chemistry, 265(13), 7693-7700 (1990-05-05)
Human single-stranded DNA binding protein (human SSB) is a multisubunit protein containing polypeptides of 70, 34, and 11 kDa that is required for SV40 DNA replication in vitro. In this report we identify the functions of the SSB and its
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