R7876
Ribonucleic acid, transfer from wheat germ
Type V, 15-19 units/mg solid
Synonyme(s) :
Transfer RNA, tRNA
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Molecular Biology
type
Type V
form
lyophilized powder
specific activity
15-19 units/mg solid
storage condition
desiccated
solubility
H2O: ~2 mg/mL, clear to hazy
storage temp.
2-8°C
Quality Level
General description
Wheat germ tRNA contains many different specific tRNA molecules.
Application
Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.
Preparation Note
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Other Notes
One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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