S7901
Stem Cell Factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, cell culture | mammalian: suitable
Synonyme(s) :
SCF, c-Kit ligand
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A propos de cet article
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
powder
Mol wt:
monomer 18.6 kDa ((165 aa))
Impurities:
≤1 EU/μgtested (LAL test)
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Laissez-nous vous aiderNom du produit
Stem Cell Factor human, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
powder
potency
≤15 ng/mL ED50
quality
endotoxin tested
mol wt
monomer 18.6 kDa ((165 aa))
packaging
pkg of 10 μg, pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μgtested (LAL test)
UniProt accession no.
storage temp.
−20°C
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Application
Stem Cell Factor human has been used in
- In vitro differentiation of equine embryonic stem cells
- Chemotaxis detection system
- Ex vivo expansion of umbilical cord blood CD34+ cells
- Cytokine therapy
Biochem/physiol Actions
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Epidermal stem cell factor (SCF) is considered as an essential mast cell growth factor and a multifunctional cytokine. SCF mediates tumor development and progression. Upregulation of the SCF gene is observed in hepatocellular carcinoma.
Physical form
Lyophilized from a 0.2 μm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Analysis Note
The proliferative activity is tested in culture using TF1 human erythroleukemic cells.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Insights into Stem Cell Factor chemotactic guidance of neural crest cells revealed by a real-time directionality-based assay
Rovasio RA, et al.
European Journal of Cell Biology, 91, 375-390 (2012)
L K Ashman
The international journal of biochemistry & cell biology, 31(10), 1037-1051 (1999-12-03)
The receptor tyrosine kinase c-Kit and its ligand Stem Cell Factor (SCF) are essential for haemopoiesis, melanogenesis and fertility. SCF acts at multiple levels of the haemopoietic hierarchy to promote cell survival, proliferation, differentiation, adhesion and functional activation. It is
Th17 cell-mediated immune responses promote mast cell proliferation by triggering stem cell factor in keratinocytes.
Cho KA, et al.
Biochemical and Biophysical Research Communications, 487(4), 856-861 (2017)
Isolation and ex vivo expansion of human umbilical cord blood-derived CD34+ stem cells and their cotransplantation with or without mesenchymal stem cells.
Delalat, B, et al.
Hematology, 14.3, 375-390 (2009)
Roberto A Rovasio et al.
European journal of cell biology, 91(5), 375-390 (2012-03-03)
The extracellular environment through which neural crest cells (NCCs) translocate and differentiate plays a crucial role in the determination of cell migration and homing. In the trunk, NCC-derived melanocyte precursor cells (MPCs) take the dorsolateral pathway and colonize the skin
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