S8633
Sphingomyelinase from Staphylococcus aureus
buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)
Synonyme(s) :
Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
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Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Specific activity:
100-300 units/mg protein (Lowry)
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buffered aqueous glycerol solution
specific activity
100-300 units/mg protein (Lowry)
storage temp.
2-8°C
Quality Level
Biochem/physiol Actions
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.
Other Notes
One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.
Physical form
Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5
Application
Sphingomyelinase from Staphylococcus aureus has been used to:
- induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline
- determine the concentration of sphingomyelin from serum samples
- enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Measurement of sphingomyelin and ceramide cellular levels after sphingomyelinase-mediated sphingomyelin hydrolysis.
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Kufor-Rakeb syndrome (KRS) is caused by loss-of-function mutations in ATP13A2 (PARK9) and characterized by juvenile-onset parkinsonism, pyramidal signs, and cognitive decline. Previous studies suggested that PARK9 deficiency causes lysosomal dysfunction and α-synuclein (α-syn) accumulation, whereas PARK9 overexpression suppresses toxicity of
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Nutrition research (New York, N.Y.), 32(12), 956-964 (2012-12-19)
We have previously reported that dietary sericin improves epidermal dryness with the increased total Ceramide (Cer) in NC/Nga mice, an animal model of atopic dermatitis (AD). In this study, we hypothesized that the increased level of total Cer induced by
Feixiang Wang et al.
The Journal of clinical investigation, 131(19) (2021-08-18)
Proper metabolic activities facilitate T cell expansion and antitumor function; however, the mechanisms underlying disruption of the T cell metabolic program and function in the tumor microenvironment (TME) remain elusive. Here, we show a zinc finger protein 91-governed (ZFP91-governed) mechanism
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