SCA20
Sucrose Assay Kit
sufficient for 20 assays
Synonyme(s) :
Sucrose Test Kit
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UNSPSC Code:
12164500
EC Number:
200-334-9
NACRES:
NA.84
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sufficient for 20 assays
Quality Level
analyte chemical class(es)
sugars (sucrose)
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
General description
Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.
Application
This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.
Composants de kit seuls
Réf. du produit
Description
- glucose assay reagent 50 mL
- sucrose assay reagent 1 mL/vial
- sucrose standard 500 mg
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
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Contenu apparenté
Instructions
Ye Ji Lee et al.
Frontiers in plant science, 12, 644455-644455 (2021-07-20)
Nitrogen is one of the most important macro-nutrients for plant growth and crop productivity. The amount of synthetic nitrogen fertilizers supplied to crops has dramatically increased, leading to a notable rise in crop yields. However, excessive nitrogen use has an
Bergmeyer, H.U. and Bernt, E.
Methods of Enzymatic Analysis, 1176-1176 (1974)
Raja S Payyavula et al.
BMC plant biology, 12, 39-39 (2012-03-21)
Plant secondary metabolites, including phenylpropanoids and carotenoids, are stress inducible, have important roles in potato physiology and influence the nutritional value of potatoes. The type and magnitude of environmental effects on tuber phytonutrients is unclear, especially under modern agricultural management