SRP3189
APRIL from mouse
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonyme(s) :
CD256, TALL-2, ZTNF2, A Proliferating-inducing Ligand, TNFSF13, TRDL-1a
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A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥98% (HPLC), ≥98% (SDS-PAGE)
Biological source:
mouse
Recombinant:
expressed in E. coli
Mol wt:
21.9 kDa
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Laissez-nous vous aiderbiological source
mouse
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
mol wt
21.9 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
Protein ID accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... TNFSF13(69583)
General description
Research area: Immunology and Cytokines
APRIL (a proliferation-inducing ligand) was identified in 1998 through database mining and is a member of the TNF (tumor necrosis factor) family. APRIL gene is localized to human chromosome 17p13, contains six exons, and is alternatively transcribed to 1.8, 2.1, and 2.4 kb mRNA transcripts. APRIL protein is composed of a cytosolic domain of 28 amino acids, and a transmembrane region. It also contains an exoplasmic region, made up of a stalk and a TNF domain. It shares the highest similarity to BLyS (B lymphocyte stimulator) protein, ~30% sequence similarity in the TNF domain. It is synthesized as a type II transmembrane protein and is proteolytically processed as a mature protein, which is a soluble and non-covalent trimer. Recombinant murine APRIL is a soluble 21.9 kDa protein, consisting of 192 amino acid residues.
APRIL (a proliferation-inducing ligand) was identified in 1998 through database mining and is a member of the TNF (tumor necrosis factor) family. APRIL gene is localized to human chromosome 17p13, contains six exons, and is alternatively transcribed to 1.8, 2.1, and 2.4 kb mRNA transcripts. APRIL protein is composed of a cytosolic domain of 28 amino acids, and a transmembrane region. It also contains an exoplasmic region, made up of a stalk and a TNF domain. It shares the highest similarity to BLyS (B lymphocyte stimulator) protein, ~30% sequence similarity in the TNF domain. It is synthesized as a type II transmembrane protein and is proteolytically processed as a mature protein, which is a soluble and non-covalent trimer. Recombinant murine APRIL is a soluble 21.9 kDa protein, consisting of 192 amino acid residues.
Biochem/physiol Actions
A proliferation-inducing ligand (APRIL) and its ligand interaction activate several signaling pathways, including nuclear factor-kappa B (NF-κB), p38, c-Jun N-terminal kinase mitogen-activated protein kinase (JNK MAPK), and Protein Kinase B/Forkhead Box O (AKT/FOXO), which are necessary for promoting the survival and differentiation of B cells and plasma cells. Additionally, APRIL signaling aids in the regulation of cytokine production, the generation of humoral immunity, decreases T cell-dependent antibody responses, and facilitates IgA class switching. These functions highlight the crucial role of APRIL in regulating immune responses. Furthermore, APRIL is also known to induce immune cell apoptosis leading to immunosuppression, and tumor development.
Physical form
Lyophilized from 10 mM Sodium Acetate, pH 5.0 + 100 mM Arginine.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MRREVSRLQR SGGPSQKQGE RPWQSLWEQS PDVLEAWKDG AKSRRRRAVL TQKHKKKHSV LHLVPVNITS KDSDVTEVMW QPVLRRGRGL EAQGDIVRVW DTGIYLLYSQ VLFHDVTFTM GQVVSREGQG RRETLFRCIR SMPSDPDRAY NSCYSAGVFH LHQGDIITVK IPRANAKLSL SPHGTFLGFV KL
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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TNFSF13 Is a Novel Onco-Inflammatory Marker and Correlates With Immune Infiltration in Gliomas
Chen R, et al.
Frontiers in immunology (2021)
Tumor necrosis factor superfamily member APRIL contributes to fibrotic scar formation after spinal cord injury.
Funk LH. et al.
Journal of Neuroinflammation, 13(1) (2016)
APRIL Drives a Coordinated but Diverse Response as a Foundation for Plasma Cell Longevity
Stephenson S, et al.
Journal of Immunology, 209(5), 926-937 (2022)
Tarlan Mamedov et al.
PloS one, 14(3), e0213438-e0213438 (2019-03-13)
A plant expression platform with eukaryotic post-translational modification (PTM) machinery has many advantages compared to other protein expression systems. This promising technology is useful for the production of a variety of recombinant proteins including, therapeutic proteins, vaccine antigens, native additives
An APRIL to remember: novel TNF ligands as therapeutic targets.
Dillon SR, et al.
Nature Reviews. Drug Discovery, 5(3), 235-246 (2006)
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