SRP5246
PAK1, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonyme(s) :
MGC130000, MGC130001, PAKα
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A propos de cet article
Specific activity:
63-85 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf9 cells
Service technique
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Laissez-nous vous aiderbiological source
human
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
63-85 nmol/min·mg
mol wt
~95 kDa
NCBI accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PAK1(5058)
General description
p21 (RAC1) activated kinase 1 (PAK1) is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3. The gene encoding this protein is localized on human chromosome 11q13.
Biochem/physiol Actions
p21-activated kinases (PAKs) are serine/threonine kinases that are activated by and are effectors of small GTPases, cell division control protein 42 (CDC42) and Ras-related C3 botulinum toxin substrate (RAC). PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix. The protein has a role in numerous cellular processes and has been shown to be expressed in squamous non-small cell lung carcinomas.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe de stockage
10 - Combustible liquids
wgk
WGK 1
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Contenu apparenté
Instructions
Essential role of CIB1 in regulating PAK1 activation and cell migration.
Leisner TM
The Journal of Cell Biology, 170(3), 465-476 (2005)
Dynamics of the Rho-family small GTPases in actin regulation and motility.
Spiering D
Cell adhesion & migration, 5(2), 170-180 (2011)
Targeting p21-activated kinase 1 (PAK1) to induce apoptosis of tumor cells.
Ong CC
Proceedings of the National Academy of Sciences of the USA, 108(17), 7177-7182 (2011)