T0198

Anti-β-Tubulin antibody, Mouse monoclonal

Name: Anti-β-Tubulin antibody, Mouse monoclonal
Brand: SIGMA

clone D66, purified from hybridoma cell culture

Synonyme(s) :

Anti-Tubulin beta-1 chain

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03792414

Nom du produit

Anti-β-Tubulin antibody, Mouse monoclonal, clone D66, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

D66, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

human, bovine, chicken, rat, canine, Chlamydomonas reinhardtii, sea urchin (Lytechinus pictus and Paracentrotus lividus), mouse, hamster, Oxyrrhis marina (dinoflagellate), rabbit, monkey

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunocytochemistry: suitable (3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.5-1.0 μg/mL using whole extract of cultured rat adrenal pheochromocytoma (PC-12) cells

isotype

IgG1

UniProt accession no.

Q9H4B7

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)
mouse ... Tubb1(545486), Tubb2a(22151), Tubb2c(227613)
rat ... Tubb2(29212), Tubb2c(296554)

Application

Monoclonal Anti-β-Tubulin antibody produced in mouse has also been used in immunofluorescence assay and enzyme linked immunosorbent assay (ELISA).

Monoclonal Anti-β-tubulin antibody produced in mouse has been used in western blotting and immunocytochemistry.

Can be used in studies involving the inhibition of flagellar motility in permeabilized sperm models.

Biochem/physiol Actions

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β- tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

β-Tubulin belongs to the subfamily of tubulins, which is the major building block of microtubules. β-tubulin has a molecular weight of 55kDa. β−Tubulin structure is characterized with core of two β -sheets enclosed by a-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (β I (class I), β II (class II), β III (class III), β IVa (class IVa), β IVb (class IVb), β V (class V), and β VI (class VI)).

Monoclonal Anti-β-Tubulin (mouse IgG1 isotype) is derived from the D66 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a sea urchin (Lytechinus pictus) sperm axonemal proteins.

Immunogen

sea urchin (Lytechinus pictus) sperm axonemal proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

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Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Expression and subcellular distribution of UNC119a, a protein partner of transducin alpha subunit in rod photoreceptors

Sinha S, et al.

Cellular Signalling, 25(1), 341-348 (2013)

Activity-Dependent Shedding of the NMDA Receptor Glycine Binding Site by Matrix Metalloproteinase 3: A PUTATIVE Mechanism of Postsynaptic Plasticity

Pauly T, et.al.

PLoS ONE, 3, e2681-e2681 (2008)

Current understanding of usher syndrome type II.

Jun Yang et al.

Frontiers in bioscience (Landmark edition), 17, 1165-1183 (2011-12-29)

Usher syndrome is the most common deafness-blindness caused by genetic mutations. To date, three genes have been identified underlying the most prevalent form of Usher syndrome, the type II form (USH2). The proteins encoded by these genes are demonstrated to

D quadrant specification in the leech Helobdella: actomyosin contractility controls the unequal cleavage of the CD blastomere.

Deirdre C Lyons et al.

Developmental biology, 334(1), 46-58 (2009-07-18)

The unequal division of the CD blastomere at second cleavage is critical in establishing the second embryonic axis in the leech Helobdella, as in other unequally cleaving spiralians. When CD divides, the larger D and smaller C blastomeres arise invariantly

Constitutive p53 heightens mitochondrial apoptotic priming and favors cell death induction by BH3 mimetic inhibitors of BCL-xL.

J Le Pen et al.

Cell death & disease, 7, e2083-e2083 (2016-02-06)

Proapoptotic molecules directly targeting the BCL-2 family network are promising anticancer therapeutics, but an understanding of the cellular stress signals that render them effective is still elusive. We show here that the tumor suppressor p53, at least in part by

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Data Sheet - T0198

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