T0678
Anti-Tryptophan Hydroxylase Antibody
mouse monoclonal, WH-3
Synonyme(s) :
Anti-TPH
Sélectionner une taille de conditionnement
A propos de cet article
Nom du produit
Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, clone WH-3, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
WH-3, monoclonal
mol wt
antigen 55 kDa
contains
15 mM sodium azide
species reactivity
human, monkey, rabbit, rat
technique(s)
immunohistochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:1,000 using rabbit pineal gland extract
isotype
IgG3
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TPH1(7166), TPH2(121278)
rat ... Tph1(24848), Tph2(317675)
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
General description
Immunogen
Application
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
- immunoblot
- immunocytochemistry
Biochem/physiol Actions
Disclaimer
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Classe de stockage
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, be used to stain mouse brain sections?
This antibody has been used successfully on mouse brain slices in an immunohistochemical application. The IHC results from an experiment employing T0678 at 1:500 in mouse brain slice staining clearly showed the dorsal nuclear raphe (which are the serotonergic neurons).
Does Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, cross-react with tyrosine or phenylalanine hydroxylases?
This antibody, unlike some other antibodies to tryptophan hydroxylases (WH) in the market, does not cross react with tyrosine hydroxylase (TH) or with phenylalanine hydroxylase (PH). The epitope recognized by the antibody is not located in the Pterin Binding Region, which is homologous among PH, TH and WH. The specificity to WH is confirmed by the single band obtained at the 55KD region, while TH has a higher MW (60-68KD), depending on the submit) and PH has a lower MW. It has also been confirmed by collaborating experts. See Journal of Neuroscience Methods, 114(2), 205-212 (2002).
Do you have an immunohistochemistry protocol for use with Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse?
Free floating sections (4% paraformaldehyde fixation)1. Incubate 30 minutes at room temperature in 0.3% Triton X-100, 5% normal serum.2. Incubate with the antibody diluted 1:10,000 in PBS, 0.3% Triton X-100, 3.5% normal serum and 0.05% BSA for 36-48 hours.3. Rinse 3 times for 5 minutes each in PBS.4. Add the biotinylated anti-mouse IgG3 diluted in PBS containing 0.3% Triton X-100 and 3.5% normal serum for 1 hour at room temperature.5. Rinse 3 times for 5 minutes each in PBS.6. Process with Avidin Biotin-HRP.7. Complete the reaction with a 2-10 minute incubation in PBS containing 0.05% DAB and 0.015% hydrogen peroxide (H2O2).8. Mount the sections on gel coated slides and air dry.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Contenu apparenté
Instructions
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique