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Merck

T2928

Anti-Tyrosine Hydroxylase Antibody

mouse monoclonal, TH-16

Synonyme(s) :

Anti-DYT14, Anti-DYT5b, Anti-TYH

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00213051

Nom du produit

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse, clone TH-16, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

TH-16, monoclonal

contains

15 mM sodium azide

species reactivity

rat, sheep, human, monkey, bovine, rabbit, guinea pig

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable using native or SDS-denatured TH.
microarray: suitable
western blot: 1:8,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells.

isotype

IgG1

UniProt accession no.

P07101

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TH(7054)
rat ... Th(25085)

Biochem/physiol Actions

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).
The antibody recognizes an epitope present in the N-terminal region (between aa 40-152) of both rodent and human tryosine hydroxylase. TH-16 detects both the intact subunits and the 59/57 kDa doublet and an array of decreasing molecular weight TH forms resulting from severe proteolysis.

Application

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in
  • western blotting
  • immunofluorescence labelling
  • immunohistochemical staining
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunoprecipitation
  • immunocytochemistry

Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in immunohistochemistry and double immunofluorescence labeling.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the TH-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Tyrosine hydroxylase (TH) exists in four isoforms in humans. They are are TH-1 (M.W. 55,600), TH-2 (4 additional amino acids; M.W. 56,000), TH-3, (27 additional amino acids; M.W. 58,100), and TH-4 (4 plus 27 additional amino acids; 58,500). Only humans express all four isoforms; all other anthropoids express only the TH-1 and TH-2 isoforms.
Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor.

Immunogen

rat tyrosine hydroxylase (TH).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000508186
0000508186
0000458293
0000458293
0000435633

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Consulter la Bibliothèque de documents

Selective cholinergic depletion of pedunculopontine tegmental nucleus aggravates freezing of gait in parkinsonian rats
Xiao H, et al.
Neuroscience Letters, 659, 92-98 (2017)
Influence of aging on the dopaminergic neurons in the substantia nigra pars compacta of rats
Gao J, et al.
Current Aging Science, 4(1), 19-24 (2011)
Álvaro García-Avilés et al.
Frontiers in neuroanatomy, 9, 33-33 (2015-04-09)
Methylphenidate (MPD) is a commonly administered drug to treat children suffering from attention deficit hyperactivity disorder (ADHD). Alterations in septal driven hippocampal theta rhythm may underlie attention deficits observed in these patients. Amongst others, the septo-hippocampal connections have long been
The astrocytic response to the dopaminergic denervation of the striatum.
Morales I, et al.
Journal of Neurochemistry, 139(1), 81-95 (2016)
Shumin Ma et al.
Oxidative medicine and cellular longevity, 2016, 4693703-4693703 (2016-05-20)
Excessive reactive oxygen species/reactive nitrogen species (ROS/RNS) produced as a result of ageing causes damage to macromolecules and organelles or leads to interference of cell signalling pathways, which in turn results in oxidative stress. Oxidative stress occurs in many neurodegenerative
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Contenu apparenté

Data Sheet - T2928

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