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Merck

T3559

Anti-γ-Tubulin Antibody

rabbit polyclonal

Synonyme(s) :

Anti-γ-Tubulin

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.46
Conjugate:
unconjugated
Clone:
polyclonal
Application:
indirect immunofluorescence
microarray
western blot
Species reactivity:
human, chicken
Citations:
177
Technique(s):
indirect immunofluorescence: 1:5,000 using methanol/acetone-fixed chicken fibroblasts
microarray: suitable
western blot: 1:1,000 using cultured A431 whole cell extracts
Uniprot accession no.:
P23258

Principaux documents

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Nom du produit

Anti-γ-Tubulin antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

human, chicken

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:5,000 using methanol/acetone-fixed chicken fibroblasts
microarray: suitable
western blot: 1:1,000 using cultured A431 whole cell extracts

UniProt accession no.

P23258

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBG1(7283), TUBG2(27175)

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Catégories apparentées

Application

Anti-γ-Tubulin antibody produced in rabbit has been used in
  • immunofluorescence analysis
  • western blotting
  • immunohistochemistry

Anti-γ-Tubulin polyclonal antibody is suitable for immunoblotting of materials such as A431 cultured cells whole extract (g-tubulin, 48 kDa), and immunocytochemical staining of cells such as cultured chicken fibroblast cells.

Biochem/physiol Actions

γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. γ-Tubulin is not a member of microtubule like α- and β-tubulin. It functions as the microtubule nucleator at the microtubule organizing centre (MTOC). It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end.
Anti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-terminally added lysine).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. It is related to α- and β-tubulin and is a member of the tubulin superfamily of proteins. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares approximately 28-32% identity with α-tubulin from various organisms and 32-36% identity with β-tubulins. Some regions are highly conserved among α-, β- and γ-tubulins.

Immunogen

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000480993
0000470985
0000470985
0000416015
0000370536

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Consulter la Bibliothèque de documents

A nice ring to the centrosome
Oakley BR
Nature, 378(6557), 555-555 (1995)
ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity
Oricchio E, et al.
Cell Cycle, 5(1), 88-92 (2006)
gamma-Tubulin-gamma-Tubulin Interactions as the Basis for the Formation of a Meshwork
Rossello C, et al.
International Journal of Molecular Sciences, 19(10), 3245-3245 (2018)
Myeloproliferative disorder FOP-FGFR1 fusion kinase recruits phosphoinositide-3 kinase and phospholipase C gamma at the centrosome
Lelievre H, et al.
Molecular Cancer, 7(1), 30-30 (2008)
Kassandra M Ori-McKenney et al.
Neuron, 76(5), 921-930 (2012-12-12)
Microtubule nucleation is essential for proper establishment and maintenance of axons and dendrites. Centrosomes, the primary site of nucleation in most cells, lose their function as microtubule organizing centers during neuronal development. How neurons generate acentrosomal microtubules remains unclear. Drosophila
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