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Merck

T4528

Thioglucosidase from Sinapis alba (white mustard) seed

≥100 units/g solid

Synonyme(s) :

Glucosinolase, Myrosinase, Thioglucoside glucohydrolase

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A propos de cet article

Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
Numéro CE :
MDL number:
Specific activity:
≥100 units/g solid
Biological source:
plant seeds (Sinapis alba)
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biological source

plant seeds (Sinapis alba)

form

solid

specific activity

≥100 units/g solid

storage temp.

−20°C

Quality Level

General description

Thioglucosidase, also called as myrosinase, is present in the myrosin cells that do not contain glucosinolates. This enzyme is obtained from several plant sources, such as Lepidium sativum, L. Sinapis alba and Brassica napus.

Application

Thioglucosidase from Sinapis alba (white mustard) seeds has been used as a standard to quantify myrosinase activity and in column glucosinolate analysis of plant samples.
Thioglucosidase has been used in a study to assess Brassica species screening for glucosinolate content. Thioglucosidase has also been used in a study to investigate a negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana.

Biochem/physiol Actions

Myrosinases are present in many bacteria, fungi, and edible plants, including those of the Brassicaceae (Cruciferae) family. The enzyme hydrolyzes the S-glucosidic bond of a glucosinolate substrate to form an unstable aglycone that rearranges with the loss of sulfate primarily to the isothiocyanate, though thiocyanates and nitriles are also formed. Many of the isothiocyanate products of aliphatic and aromatic glucosinolates have cancer chemopreventive properties.
Thioglucosidase research has focused mainly on the cruciferous crops due to their economic importance and cancer preventive benefits.

Other Notes

One unit will produce 1.0 μmole glucose per min from sinigrin at pH 6.0 at 25 °C.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Teresa Oliviero et al.
Molecular nutrition & food research, 62(18), e1700837-e1700837 (2018-03-14)
Optimization of bioavailability of dietary bioactive health-beneficial compounds is as important as increasing their concentration in foods. The aim of this study is to explore the change in bioavailability of isothiocyanates (ITCs) in broccoli sprouts incorporated in protein, fiber, and
George F Antonious et al.
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 44(3), 311-316 (2009-03-13)
Glucosinolates (GSLs), a group of compounds found in Brassica plants, are toxic to some soil-borne plant pathogens because of the toxicity of their hydrolysis products, isothiocyanates. Other phytochemicals found in Brassica plants, such as phenols and ascorbic acid, may compliment
Niels Agerbirk et al.
Phytochemistry, 153, 79-93 (2018-06-11)
Glucosinolates are found in plants of the order Brassicales and hydrolyzed to different breakdown products, particularly after tissue damage. In Barbarea vulgaris R.Br. (Brassicaceae), the dominant glucosinolate in the investigated "G-type" is glucobarbarin, (S)-2-hydroxy-2-phenylethylglucosinolate. Formation of the nitrile from glucobarbarin
Wei Chen et al.
Frontiers in physiology, 13, 1013092-1013092 (2022-11-08)
Glycoside hydrolase family 1 (GH1) members exhibit a broad substrate spectrum and play important roles in insect-plant interactions, such as the defensive β-glucosidase and β-thioglucosidase (so-called myrosinase). However, knowledge about the expression profiling and function of glycoside hydrolase family 1
Niels Agerbirk et al.
Phytochemistry, 153, 79-93 (2018-06-11)
Glucosinolates are found in plants of the order Brassicales and hydrolyzed to different breakdown products, particularly after tissue damage. In Barbarea vulgaris R.Br. (Brassicaceae), the dominant glucosinolate in the investigated "G-type" is glucobarbarin, (S)-2-hydroxy-2-phenylethylglucosinolate. Formation of the nitrile from glucobarbarin

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