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T5201

Anti-β-Tubulin antibody, Mouse monoclonal

clone TUB 2.1, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-β-Tubulin

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A propos de cet article

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
TUB 2.1, monoclonal
Application:
ARR, IEP, IF, IHC, WB
Citations:
278
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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TUB 2.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

indirect immunofluorescence: 10-20 μg/mL using cultured chicken fibroblasts, microarray: suitable, immunoelectrophoresis: suitable, immunohistochemistry: suitable, western blot: 2-4 μg/mL using chicken fibroblasts cell extract

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383)
mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152)
rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)

General description

β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-Tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βI (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).
Monoclonal Anti-β-Tubulin (mouse IgG1 isotype) is derived from the hybridoma TUB 2.1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified rat brain tubulin.

Immunogen

tubulin from rat brain.

Application

Monoclonal Anti-β-Tubulin antibody produced in mouse has also been used in
  • 2-dimensional electrophoresis
  • sperm motility
  • immunohistochemistry,
  • immunofluorescence
  • immunoblotting
  • immunocytochemistry

Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting.

Biochem/physiol Actions

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).
The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificats d'analyse (COA)

Lot/Batch Number
0000527531
0000527531
0000519680
0000519680
0000457889

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Contenu apparenté

Involvement of argonaute proteins in gene silencing and activation by RNAs complementary to a non-coding transcript at the progesterone receptor promoter.
Chu Y
Nucleic Acids Research, 38(21), 7736-7748 (2010)
Shuqun Yang et al.
PloS one, 8(8), e71697-e71697 (2013-08-27)
The protein TIN2 is a member of telomere-binding protein complex that serves to cap and protect mammalian chromosome ends. As a number of proteins in this complex are phosphorylated in a cell cycle-dependent manner, we investigated whether TIN2 is modified
Beta-tubulin binds Src homology 2 domains through a region different from the tyrosine-phosphorylated protein-recognizing site.
Itoh T
The Journal of Biological Chemistry, 271(44), 27931-27935 (1996)
Afficher tous les articles scientifiques apparentés