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Merck

T5201

Anti-β-Tubulin antibody, Mouse monoclonal

clone TUB 2.1, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-β-Tubulin

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Nom du produit

Anti-β-Tubulin antibody, Mouse monoclonal, clone TUB 2.1, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TUB 2.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunoelectrophoresis: suitable
immunohistochemistry: suitable
indirect immunofluorescence: 10-20 μg/mL using cultured chicken fibroblasts
microarray: suitable
western blot: 2-4 μg/mL using chicken fibroblasts cell extract

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383)
mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152)
rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)

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General description

Monoclonal Anti-β-Tubulin (mouse IgG1 isotype) is derived from the hybridoma TUB 2.1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified rat brain tubulin.
β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-Tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βI (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).

Immunogen

tubulin from rat brain.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Application

Monoclonal Anti-β-Tubulin antibody produced in mouse has also been used in
  • 2-dimensional electrophoresis
  • sperm motility
  • immunohistochemistry,
  • immunofluorescence
  • immunoblotting
  • immunocytochemistry

Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting.

Biochem/physiol Actions

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).
The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000519680
0000519680
0000457889
0000457889
0000435635

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Consulter la Bibliothèque de documents

Involvement of argonaute proteins in gene silencing and activation by RNAs complementary to a non-coding transcript at the progesterone receptor promoter.
Chu Y
Nucleic Acids Research, 38(21), 7736-7748 (2010)
Shuqun Yang et al.
PloS one, 8(8), e71697-e71697 (2013-08-27)
The protein TIN2 is a member of telomere-binding protein complex that serves to cap and protect mammalian chromosome ends. As a number of proteins in this complex are phosphorylated in a cell cycle-dependent manner, we investigated whether TIN2 is modified
Structure of the alpha beta tubulin dimer by electron crystallography.
Nogales E
Nature, 391(6663), 199-203 (1998)
Kai Jiang et al.
Developmental cell, 28(3), 295-309 (2014-02-04)
Microtubules are cytoskeletal polymers with two structurally and functionally distinct ends, the plus- and the minus-end. Here, we focus on the mechanisms underlying the regulation of microtubule minus-ends by the CAMSAP/Nezha/Patronin protein family. We show that CAMSAP2 is required for
HLA-B2702 (77-83/83-77) peptide binds to beta-tubulin on human NK cells and blocks their cytotoxic capacity.
Tarazona R
Journal of Immunology, 165(12), 6776-6782 (2000)
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