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Merck

T9285

Tampon Tris-EDTA

100X concentrate for Northern and Southern blotting

Synonyme(s) :

Tampon TE solution

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A propos de cet article

NACRES:
NA.25
PubChem Substance ID:
329826904
UNSPSC Code:
41105319
MDL number:
MFCD00236359

Principaux documents

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Nom du produit

Tampon Tris-EDTA, 100X concentrate for Northern and Southern blotting

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

grade

Molecular Biology

sterility

0.2 μm filtered

form

liquid

pH

7.8-8.2 (1 × in water)

application(s)

sample preparation

foreign activity

DNase, RNase, none detected

storage temp.

room temp

Quality Level

200

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Catégories apparentées

Application

TE buffer has been used for:
  • isolate, resuspend, or store DNA and RNA for PCR or qPCR
  • gel electrophoresis, including Northern and Southern blotting
  • bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
  • antigen retrieval for immunohistochemistry

General description

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Preparation Note

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Classe de stockage

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000390095
0000390095
0000443464
0000443464
SLCN3561

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?

    When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.

  6. What is the pH and color of Product T9285, Tris-EDTA buffer solution?

    The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.

  7. What is the shelf life of Product T9285, Tris-EDTA buffer solution?

    This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.

  8. What does 'for molecular biology' mean?

    The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Black JA, et al.
Journal of Clinical Microbiology, 43, 3110-3113 (2005)
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Experimental and therapeutic medicine, 9(3), 901-904 (2015-02-11)
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Germline epigenetic factors influence transgenerational inheritance of behavioral traits upon changes in experience and environment. Immune activation due to infection can also modulate brain function, but whether this experience can be passed down to offspring remains unknown. Here, we show
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Immunology and cell biology, 94(2), 185-195 (2015-08-14)
Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel
Jennifer A Black et al.
Journal of clinical microbiology, 43(7), 3110-3113 (2005-07-08)
Although plasmid-mediated AmpC beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. These enzymes are typically produced by isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. and are
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