TOX6
In Vitro Toxicology Assay Kit, Sulforhodamine B based
Synonyme(s) :
biomass viability assay, sulforhodamine B, total biomass assay
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A propos de cet article
NACRES:
NA.84
UNSPSC Code:
12352207
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liquid
usage
kit sufficient for 1,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
565 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
room temp
Quality Level
General description
The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.
Application
In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.
Biochem/physiol Actions
Dye binds to cellular protein and is then solubilized in base.
signalword
Danger
wgk
WGK 2
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
Classe de stockage
8A - Combustible corrosive hazardous materials
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P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
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Evaluation of colorimetric protein and biomass stains for assaying drug effects upon human tumor cell lines.
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Proceedings of the American Association For Cancer Research, 30, 2436-2436 (1989)
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Recent data have redefined the concept of inflammation as a critical component of tumor progression. However, there has been little development on cases where inflammation on or near a wound and a tumor exist simultaneously. Therefore, this pilot study aims
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