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Merck

TP0100

Total Protein Kit, Micro

Synonyme(s) :

Bradford

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A propos de cet article

NACRES:
NA.32
UNSPSC Code:
12352202
Service technique
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description

wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L

Quality Level

parameter

18-26 °C temperature, 50 μL sample volume

storage temp.

2-8°C

General description

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Application

Colorimetric, Endpoint
Suitable for protein determination for samples of up to 0.5 mg/ml.
Total protein kit, micro has been used to determine:
  • total protein in the bacterial homogenate by Bradford method
  • total protein content by the Bradford microassay from the tissue extracts
  • serum total protein
  • soluble protein content by the Bradford method from leaves

Biochem/physiol Actions

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.
Total protein kit, micro can be used to determine the concentration of proteins in solution.


Composants de kit également disponibles séparément

Réf. du produit
Description
FDS & Tarif

  • Protein Standard 5 mL
    FDS

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

target_organs

Eyes,Central nervous system

Classe de stockage

3 - Flammable liquids

flash_point_f

132.8 °F

flash_point_c

56 °C

wgk

WGK 3



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Contenu apparenté


Zeynep Özdemir et al.
Pharmacological reports : PR, 72(3), 692-704 (2020-03-08)
Since brain neurotransmitter levels are associated with the pathology of various neurodegenerative diseases like Parkinson and Alzheimer, monoamineoxidase (MAO) plays a critical role in balancing these neurotransmitters in the brain. MAO isoforms appear as promising drug targets for the development
Elizabeth Gray et al.
Journal of neuropathology and experimental neurology, 67(9), 888-899 (2008-08-22)
Matrix metalloproteinases (MMPs) degrade extracellular matrix; MMP activity, particularly of MMP-9, is elevated in the white matter in multiple sclerosis (MS) patients. The cerebral cortical extracellular matrix includes perineuronal nets (PNs) that surround parvalbumin-positive neurons (PV-positive neurons) and are important
Francisca P Martínez-Antequera et al.
Animals : an open access journal from MDPI, 11(6) (2021-07-03)
This study evaluated the use of Ulva ohnoi as an ingredient in feeds for aquaculture in three different experiments. Experiment 1 was oriented to confirm the negative effect of U. ohnoi on fish digestion. Experiment 2 assessed the effect on