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V4888

Anti-VSV-G antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-Vesicular Stomatitis Virus glycoprotein

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
MFCD00164761
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
73
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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mammals

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates), indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells, western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates

shipped in

dry ice

storage temp.

−20°C

General description

The Anti-VSV-G rabbit antibody reacts with amino- and carboxyl-terminal VSV-G tagged fusion proteins.
Vesicular stomatitis viruses (VSV) belongs to the Vesiculovirus genus of the Rhabdoviridae family. Vesicular stomatitis virus G (VSV G) protein is considered to be the early recognized type III viral fusion protein.

Immunogen

synthetic peptide corresponding to amino acids 497-511 (N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C) of the Vesicular Stomatitis Virus glycoprotein (VSV-G), conjugated to maleimide activated KLH via a cysteine residue added at the carboxyl terminus. The antibody recognizes the VSV-G tag at both the C-terminus and N-terminus.

Application

Antibody is suitable for immunoblotting, immunoprecipitation, and indirect immunofluorescence.
immunocytochemistry.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Western Blotting (1 paper)
Western blotting following immunoprecipitation (1 paper)
Anti-VSV-G antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Biochem/physiol Actions

Vesicular stomatitis virus G (VSV G) is capable of stimulating cell-cell fusion upon low pH stimulation. The transmembrane region of VSV G protein is highly essential for the virus transition from the hemi-fusion to full fusion state.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

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Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000515686
0000515686
0000453928
0000453928
0000414612

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Contenu apparenté

Data Sheet - V4888

Instructions

Vesicular stomatitis virus glycoprotein is a determinant of pathogenesis in swine, a natural host
Martinez I, et al.
Journal of virology, 77(14), 8039-8047 (2003)
Vesicular stomatitis virus G protein transmembrane region is crucial for the hemi-fusion to full fusion transition
Ci Y, et al.
Scientific Reports, 8(1), 10669-10669 (2018)
Periodic fever with aphthous stomatitis, pharyngitis, and cervical adenitis syndrome is associated with a CARD8 variant unable to bind the NLRP3 inflammasome
Cheung MS, et al.
Journal of immunology (Baltimore, Md. : 1950), 198(5), 2063-2069 (2017)
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