Accéder au contenu
Merck

V5265

Crystal Violet Solution

1%, aqueous solution

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

A propos de cet article

Formule empirique (notation de Hill) :
C25H30ClN3
Numéro CAS:
548-62-9
Poids moléculaire :
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329829057
MDL number:
MFCD00011750
Beilstein/REAXYS Number:
3580948

Principaux documents

Voir toute la documentation
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

Crystal Violet Solution, 1%, aqueous solution

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

form

aqueous solution

concentration

1%

technique(s)

microbe id | staining: suitable

color

deep violet, purple

Amax

0.36-0.44 at 589-594 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Application

  • Crystal violet is commonly used in Gram staining for the classification of bacteria.
  • It has also been used to detect bacterial adherence to biomedical polymers and to stain DNA in mammalian tissues in Giemsa staining.
  • It has been successfully used to develop a counterion-staining method to detect DNA in agarose gel electrophoresis.
  • Its antimicrobial properties have facilitated its use in the treatment of oral candidiasis, skin infections, and methicillin-resistant Staphylococcus aureus.
  • It has been used as a stain in cell proliferation assays, migration assays, and Boyden chamber assay.
Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.

Biochem/physiol Actions

Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms entrapped complexes with Gram′s iodine that retain the purple color post-decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner.
The antimicrobial effects of crystal violet can be attributed to free radical generation and formation of unionized bacterial complexes with the dye. It has also been reported to inhibit protein synthesis and bacterial cell wall formation. It is especially effective against Gram-positive bacteria.

General description

Crystal violet, also known as gentian violet or methyl violet, is the N-hexamethylated derivative of pararosaniline. It is a basic, cationic, acidotropic protein dye used in histological and bacteriological staining. It is well-known for its antibacterial and antifungal properties.

It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000524579
0000524578
0000524575
0000524578
0000524575

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Characterization of potent and selective iodonium-class inhibitors of NADPH oxidases
Lu J, et al.
Biochemical Pharmacology, 43, 25-38 (2017)
K Engbaek et al.
Journal of clinical pathology, 32(2), 187-190 (1979-02-01)
Five techniques for Gram staining bacteria in paraffin sections were compared on serial sections of pulmonary tissues from eight bacteriological necropsies. Brown and Hopp's method was the most satisfactory for distinguishing Gram-positive and Gram-negative bacteria. However, this method cannot be
Histological and histochemical methods: Theory and practice (5th ed.)
JA Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
Studies on adsorption of crystal violet dye from aqueous solution onto coniferous pinus bark powder (CPBP)
Ahmad R.
Journal of Hazardous Materials, 171, 767-773 (2009)
Milly M Choy et al.
Cell reports, 31(6), 107617-107617 (2020-05-14)
The molecular basis of dengue virus (DENV) attenuation remains ambiguous and hampers a targeted approach to derive safe but nonetheless immunogenic live vaccine candidates. Here, we take advantage of DENV serotype 2 PDK53 vaccine strain, which recently and successfully completed
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique