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V9264

Anti-Vinculin Antibody

mouse monoclonal, hVIN-1

Synonyme(s) :

Monoclonal Anti-Vinculin antibody produced in mouse

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00162905
Conjugate:
unconjugated
Clone:
hVIN-1, monoclonal
Application:
ELISA (i), ICC, IHC, WB
Citations:
306
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Nom du produit

Anticorps monoclonal anti-vinculine antibody produced in mouse, clone hVIN-1, purified from hybridoma cell culture

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

hVIN-1, monoclonal

form

buffered aqueous solution

mol wt

antigen 116 kDa

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 0.05-0.1 μg/mL using HS-68 total cell extract

isotype

IgG1

UniProt accession no.

P18206

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

General description

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.

Immunogen

Vinculine d'utérus humain purifiée.

Application

Anti-Vinculin antibody, Mouse monoclonal has been used in
  • immunoblotting
  • immunofluorescence staining
  • immunocytochemistry
  • immunohistochemistry and enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Marquage spécifique de la vinculine au niveau des contacts cellule/cellule et cellule/substrat. Forte réactivité avec la vinculine humaine. Réactivité croisée avec la métavinculine de muscle lisse.
Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de stockage

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000532297
0000532297
0000487226
0000487227
0000487227

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Contenu apparenté

Biomimetic calcium carbonate concentric microgrooves with tunable widths for promoting MC3T3-E1 cell functions
Wu X and Wang S
Advanced Helathcare Materials, 2(2), 326-333 (2013)
Podosomes as smart regulators of cellular adhesion
Spinardi L and Marchisio PC
European Journal of Cell Biology, 85(3-4), 191-194 (2006)
Loredana Pellegrino et al.
Nucleic acids research, 41(10), 5400-5412 (2013-04-13)
Uncontrolled cell proliferation and cytoskeletal remodeling are responsible for tumor development and ultimately metastasis. A number of studies have implicated microRNAs in the regulation of cancer cell invasion and migration. Here, we show that miR-23b regulates focal adhesion, cell spreading
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