WGA2
Kit d′amplification complète de génome entier (WGA) GenomePlex®
Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue
Synonyme(s) :
Whole genome amplification kit
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Nom du produit
Kit d′amplification complète de génome entier (WGA) GenomePlex®, Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue
technique(s)
whole genome amplification: suitable
shipped in
wet ice
storage temp.
−20°C
Quality Level
Catégories apparentées
Application
- analyse sur puces à ADN
- analyse de polymorphismes mononucléotidiques (SNP)
- analyse de polymorphismes mononucléotidiques (STR)
- archivage de l'ADN
Features and Benefits
- Meilleur rendement avec une quantité minimale de matrice : amplification de quelques nanogrammes d'ADN génomique donnant un rendement de plusieurs microgrammes (environ 10 μg) en moins de trois heures environ
- Possibilité de conserver les quelques nanogrammes d'échantillons à –20 °C en vue d'une utilisation ultérieure
- Diverses sources d'ADN au choix : sang total, frottis buccal, sang sur papier buvard, plantes, sol ou tissus fixés au formol et inclus en paraffine
- Plus grande précision d'amplification grâce à l'ADN polymérase "WGA"
- Représentation du génome entier sans biais allélique détectable
- Kit compatible avec de nombreuses applications en aval : tests TaqMan®, analyse des polymorphismes mononucléotidiques ("Single Nucleotide Polymorphism", SNP), analyse par hybridation génomique comparative ("Comparative Genomic Hybridization", CGH), etc.
General description
Legal Information
Other Notes
Composants de kit également disponibles séparément
- W4502Water, Nuclease-Free Water, for Molecular Biology
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the major difference between WGA1 and WGA2 kits?
Functionally, WGA1 and WGA2 kits are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.
If starting with fragmented DNA, what is the smallest size fragment which can be successfully amplified with Product WGA2, GenomePlex® Amplification Kit, and do I still need to do the fragmentation step?
The kit works best for fragments 400 bp and larger. When starting with fragmented DNA, we recommend: (1) skipping the fragmentation heat step, although the buffer should be added, and (2) increasing the PCR cycles from 14 to 20.
Can I use Product WGA2, GenomePlex® Amplification Kit, to amplify DNA from a single cell?
Using this kit to amplify DNA from a single cell is not recommended. We recommend using the GenomePlex Single Cell WGA Kit (WGA4) for such application. WGA4 was developed for use with single cells and includes an optimized cell lysis protocol which has been incorporated into the fragmentation step.
Can I amplify single stranded DNA with WGA2, GenomePlex® Amplification Kit?
When starting with single stranded starting materials, we recommend (1) skipping the fragmentation heat step, although the buffer should be added and (2) increasing the PCR cycles from 14 to 20. Note that if the ssDNA is actually cDNA from polyadenylated RNA, the kit will likely not give good representation of the input material, as the poly(T) ends constitute a large, non-random fraction.
Is the WGA2 GenomePlex® Amplification Kit polymerase compatible with TA cloning?
WGA polymerase is compatible with TA cloning with the following alteration to the PCR step: Be sure to include a 7 to 30 minute extension at 72°C after the last cycle to ensure that all PCR products are full length and 3´ adenylated.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Instructions
Our commitment to the Life Sciences continues through significant advances in PCR technology. Improvements to the key parameters of yield, length, specificity, and fidelity, as well as new applications for nucleic acid amplification form the basis of our research and development focus. We are dedicated to providing you with the newest technology as the field of Nucleic Acid Amplification evolves, making Sigma your preferred supplier for PCR products.
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