YEAST1
Yeast Transformation Kit
reagents for introducing plasmid DNA into yeast
Synonyme(s) :
lithium acetate yeast transformation
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A propos de cet article
NACRES:
NA.85
UNSPSC Code:
12352200
Grade:
Molecular Biology
Technique(s):
transformation: suitable
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Molecular Biology
technique(s)
transformation: suitable
shipped in
dry ice
storage temp.
−20°C
Quality Level
usage
kit sufficient for >100 standard transformations
Catégories apparentées
General description
Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.
Application
Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.
Biochem/physiol Actions
Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).
Features and Benefits
- Easy and ready-to-use
- Requires as little as 10 ng of plasmid DNA
- Flexibility for any strain of yeast
- Sufficient for over 100 standard transformations
Other Notes
The Yeast Transformation Kit contains:
- Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
- Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
- Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
- Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
- Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g
Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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James R Petrie et al.
PloS one, 7(4), e35214-e35214 (2012-04-24)
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FEMS microbiology letters, 272(1), 114-119 (2007-06-22)
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DMSO-enhanced whole cell yeast transformation.
J Hill et al.
Nucleic acids research, 19(20), 5791-5791 (1991-10-25)
Miren Zumárraga et al.
Proteins, 71(1), 250-260 (2007-10-13)
The generation of diversity for directed protein evolution experiments shows an important bottleneck in the in vitro random mutagenesis protocols. Most of them are biased towards specific changes that eventually confer a predicted and conservative mutational spectrum, limiting the exploration
S Camarero et al.
Applied and environmental microbiology, 78(5), 1370-1384 (2012-01-03)
While the Pycnoporus cinnabarinus laccase (PcL) is one of the most promising high-redox-potential enzymes for environmental biocatalysis, its practical use has to date remained limited due to the lack of directed evolution platforms with which to improve its features. Here
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