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113200 Anti-α-Actin Mouse mAb (1A4)

This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

Anti-α-Actin Mouse mAb (1A4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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113200
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Bab, H, RbMMonoclonal Antibody

Pricing & Availability

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113200-500ULCN
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      Description
      OverviewRecognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
    • Antibody Target Gene Symbol: ACTA2
    • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
    • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
    • Hu Entrez ID: 59 (Related Antibodies: 113200)
    • Rat Entrez ID: 81633
      		•
      Catalogue Number113200
      Brand Family Calbiochem®
      References
      ReferencesRannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532.
      Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315.
      Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
      Negative controlFibroblasts, striated muscle, or myocardium
      Positive controlSmooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Paraffin Sections
      Application NotesFrozen Sections (1:50-1:75)
      Immunoblotting (see comments)
      Paraffin Sections (1:50-1:75)
      Application CommentsDoes not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
      ImmunogenHuman Smooth Muscle
      Clone1A4
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Baboon
      • Human
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      113200-500ULCN 04055977208054

      Documentation

      Anti-α-Actin Mouse mAb (1A4) Certificates of Analysis

      TitleLot Number
      113200

      References

      Reference overview
      Rannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532.
      Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315.
      Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

      Brochure

      Title
      Antibody Sourcebookﾙ Sixth Edition
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-July-2007 RFH
      ApplicationFrozen Sections (1:50-1:75)
      Immunoblotting (see comments)
      Paraffin Sections (1:50-1:75)
      DescriptionMouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
      BackgroundActin is a cytoskeletal protein. Smooth muscle actin forms smaller, less ordered contractile units than actin from skeletal or cardiac muscle. The activity and state of assembly of smooth muscle actin is controlled by calcium-regulated phosphorylation of one of the myosin light chains.
      HostMouse
      Immunogen speciesHuman Smooth Muscle
      Immunogena synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
      Clone1A4
      IsotypeIgG2a
      Speciesbaboon, human, rabbit
      Positive controlSmooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
      Negative controlFibroblasts, striated muscle, or myocardium
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesRannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532.
      Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315.
      Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.