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217590 Anti-CD40 Mouse mAb (EA-5)

Anti-CD40, mouse monoclonal, clone EA-5, recognizes ~44 kDa CD40 in Raji cells. It is validated for use in Flow Cytometry, immunoprecipitation, & immunohistochemistry on frozen & paraffin sections.

Anti-CD40 Mouse mAb (EA-5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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217590
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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217590-100UGCN
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~44 kDa CD40 protein in Raji cells.
      Catalogue Number217590
      Brand Family Calbiochem®
      References
      ReferencesFanslow, W.C., et al. 1992. J. Immunol. 149, 655.
      Law, C.L., et al. 1990. Leukemia 4, 732.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.
      Positive controlRaji cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoprecipitation
      Paraffin Sections
      Application NotesFlow cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Paraffin Sections (see comments)
      Application CommentsFACS was performed on 5x105 cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogentransfected murine L cells expressing human CD40
      ImmunogenHuman
      CloneEA-5
      HostMouse
      Purity≥95% by SDS-PAGE
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      217590-100UGCN 04055977219425

      Documentation

      Anti-CD40 Mouse mAb (EA-5) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD40 Mouse mAb (EA-5) Certificates of Analysis

      TitleLot Number
      217590

      References

      Reference overview
      Fanslow, W.C., et al. 1992. J. Immunol. 149, 655.
      Law, C.L., et al. 1990. Leukemia 4, 732.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-December-2008 JSW
      ApplicationFlow cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Paraffin Sections (see comments)
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~44 kDa CD40 protein.
      BackgroundHuman CD40 is a member of the tumor necrosis factor (TNF) family of cell surface receptors, and is found on all B cells (except plasma cells) and some epithelial, carcinoma, and lymphoid dendritic cells. Through binding of its ligand (CD40L/CD154/gp39), CD40 plays an important role in B-cell activation and isotype switching.
      HostMouse
      Immunogen speciesHuman
      Immunogentransfected murine L cells expressing human CD40
      CloneEA-5
      IsotypeIgG₁
      Specieshuman
      Positive controlRaji cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Preservative≤0.1% sodium azide
      CommentsFACS was performed on 5x105 cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesFanslow, W.C., et al. 1992. J. Immunol. 149, 655.
      Law, C.L., et al. 1990. Leukemia 4, 732.