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217605 Anti-CD54 Mouse mAb (15.2)

Anti-CD54, mouse monoclonal, clone 15.2, recognizes D1 domain of the ~90 kDa CD54 (ICAM-1) in Raji cells. It is validated for FC, WB, IP, IHC on frozen sections, and neutralization studies.

Anti-CD54 Mouse mAb (15.2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ICAM-1, Domain 1

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217605
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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      Description
      OverviewRecognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.
      Catalogue Number217605
      Brand Family Calbiochem®
      SynonymsAnti-ICAM-1, Domain 1
      References
      ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
      Berendt, A.R., et al. 1992. Cell 68, 71.
      Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Positive controlRaji cells
      Preservative0.5 mg/ml gentamicin sulfate
      Quality LevelMQ100
      Applications
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Neutralization Studies
      Application NotesFlow Cytometry (1-10 µg/ml)
      Frozen Sections (see comments)
      Immunoblotting (see comments)
      Immunoprecipitation (see comments)
      Neutralization Studies (see comments)
      Application CommentsFACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman monocytes
      ImmunogenHuman
      Clone15.2
      HostMouse
      Purity≥95% by SDS-PAGE
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      217605-100UGCN 04055977202892

      Documentation

      Anti-CD54 Mouse mAb (15.2) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD54 Mouse mAb (15.2) Certificates of Analysis

      TitleLot Number
      217605

      References

      Reference overview
      Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
      Berendt, A.R., et al. 1992. Cell 68, 71.
      Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      SynonymsAnti-ICAM-1, Domain 1
      ApplicationFlow Cytometry (1-10 µg/ml)
      Frozen Sections (see comments)
      Immunoblotting (see comments)
      Immunoprecipitation (see comments)
      Neutralization Studies (see comments)
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).
      BackgroundHuman CD54 (Intercellular Adhesion Molecule-1, or ICAM-1) is a ~90 kDa lymphocyte/monocyte cell surface glycoprotein that mediates cell-cell adhesion by interacting with the integrins LFA-1 (CD11a/CD18) and MAC-1 (CD11b/CD18).
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman monocytes
      Clone15.2
      IsotypeIgG₁
      Specieshuman
      Positive controlRaji cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Purity≥95% by SDS-PAGE
      Preservative0.5 mg/ml gentamicin sulfate
      CommentsFACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
      Berendt, A.R., et al. 1992. Cell 68, 71.
      Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.