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681505 Anti-CaV2.2 (851-867) Rabbit pAb

Anti-CaV2.2 (851-867), Rabbit polyclonal, recognizes the ~210 and ~240 kDa forms of α1B-subunit of Cav2.2. It is validated for use in WB, ICC, IHC, and IP.

Anti-CaV2.2 (851-867) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-N-Type-Ca2+ Channel

681505
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      M, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~210 kDa and the ~240 kDa forms of α1B-subunit of Cav2.2. Supplied with a control peptide.
      Note: The α-subunit is highly sensitive to proteases. Please refer to the product insert for proper sample preparation.
      Catalogue Number681505
      Brand Family Calbiochem®
      SynonymsAnti-N-Type-Ca2+ Channel
      Application Data
      Detection of rat CaV2.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:200). Detection: chemiluminescence.

      Detection of mouse of CaV2.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV2.2 channel (red) appearing in Purkinje cells (arrows) and distributed diffusely in the molecular layer (Mol) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) demonstrates the restruction of CaV2.2 to cell bodies but not to dendrites in the molecular layer (Figure B). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:100). Detection: fluorescence.
      References
      ReferencesWestenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
      Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
      Product Information
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 5% sucrose, 1% BSA, pH. 7.4 and 40 µg control peptide lyophilized from PBS.
      Preservative≤0.1% sodium azide (antibody only)
      Quality LevelMQ100
      Applications
      Application ReferencesImmunocytochemistry and Immunohistochemistry Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403. Immunoprecipitation Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunohistochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1.5-3 µg/ml)
      Immunocytochemistry (see application references)
      Immunohistochemistry (1:1000; see application references)
      Immunoprecipitation (see application references)
      Application CommentsThe epitope is specific for the α1B-subunit of the voltage-gated Ca2+ channel and is not present in any other known protein. Antibody binding can be fully inhibited with the soluble CP851-867 blocking peptide. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml) (Westenbroek, R.E., et al. 1995.). To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 minutes) to remove any aggregates. For use as a negative control, pre-incubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide [(C)RHHRHRDRDKTSASTPA] corresponding to amino acids 851-867 of the α1B-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDissolve the antibody in 50 µl dH₂O to make a 300 µg/ml stock solution, aliquot and freeze (-20°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Dissolve the peptide in 100 µl dH₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      681505 0

      Documentation

      Anti-CaV2.2 (851-867) Rabbit pAb Certificates of Analysis

      TitleLot Number
      681505

      References

      Reference overview
      Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
      Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-November-2009 RFH
      SynonymsAnti-N-Type-Ca2+ Channel
      ApplicationImmunoblotting (1.5-3 µg/ml)
      Immunocytochemistry (see application references)
      Immunohistochemistry (1:1000; see application references)
      Immunoprecipitation (see application references)
      Application Data
      Detection of rat CaV2.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:200). Detection: chemiluminescence.

      Detection of mouse of CaV2.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV2.2 channel (red) appearing in Purkinje cells (arrows) and distributed diffusely in the molecular layer (Mol) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) demonstrates the restruction of CaV2.2 to cell bodies but not to dendrites in the molecular layer (Figure B). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:100). Detection: fluorescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~210 kDa and the ~240 kDa forms the voltage-gated Ca2+ channel of α1B-subunit. Supplied with a control peptide (immunogen).
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide [(C)RHHRHRDRDKTSASTPA] corresponding to amino acids 851-867 of the α1B-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
      IsotypeIgG
      Speciesmouse, rat
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 5% sucrose, 1% BSA, pH. 7.4 and 40 µg control peptide lyophilized from PBS.
      Preservative≤0.1% sodium azide (antibody only)
      CommentsThe epitope is specific for the α1B-subunit of the voltage-gated Ca2+ channel and is not present in any other known protein. Antibody binding can be fully inhibited with the soluble CP851-867 blocking peptide. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml) (Westenbroek, R.E., et al. 1995.). To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 minutes) to remove any aggregates. For use as a negative control, pre-incubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsDissolve the antibody in 50 µl dH₂O to make a 300 µg/ml stock solution, aliquot and freeze (-20°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Dissolve the peptide in 100 µl dH₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesWestenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
      Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
      Application referencesImmunocytochemistry and Immunohistochemistry Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403. Immunoprecipitation Westenbroek, R.E., et al. 1992. Neuron 9, 1099.