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MABT366 Anti-Claudin-1/CLDN1 Antibody, clone 7A5

This Anti-Claudin-1 Antibody, clone 7A5 is validated for use in Immunocytochemistry, Western Blotting, Flow Cytometry for the detection of CLDN1.

Anti-Claudin-1/CLDN1 Antibody, clone 7A5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Infectious Diseases - Viral Gene Symbol: CLDN1, CLD1, ILVASC, NISCH, SEMP1, UNQ481/PRO944 UniProt Number: O95832
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MABT366
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WB, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT366
      DescriptionAnti-Claudin-1/CLDN1 Antibody, clone 7A5
      Alternate Names
      • Claudin-1
      • Senescence-associated epithelial membrane protein
      Background InformationClaudin-1 (UniProt O95832; also known as Senescence-associated epithelial membrane protein) is encoded by the CLDN1 (also known as CLD1, ILVASC, NISCH, SEMP1) gene (ORF UNQ481/PRO944; Gene ID 9076) in human. Tight junctions (TJs) are highly specialized membrane barriers that block the free passage of small molecules, microorganisms, and cells across the paracellular space, as well as provide a physical barrier between the apical and basolateral membrane domains of a polarized epithelial cell. Claudins are TJ proteins whose main function is to regulate the passage of ions across the paracellular space. There exist at least 27 human claudins with differential epithelial expression patterns, allowing tissue-specific paracellular transport of ions. Claudin-1 is expressed on apical and basolateral surfaces of hepatocytes in normal liver tissue. Claudin-1 is a known host factor for hepatitis C virus (HCV) entry via interaction with CD81 and cell-to-cell HCV transmission. Claudin-1 is a four-transmembrane (a.a. 8-28, 82-102, 116-136, 164-184) protein, having both its N- and C-terminal ends exposed cytoplasmically (a.a. 1-7 & 185-211).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Claudin-1 Antibody, clone 7A5 is validated for use in Immunocytochemistry, Western Blotting, Flow Cytometry for the detection of CLDN1.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 5.0 µg/mL from a representative lot immunostained HT1080 cells expressing exogenously transfected human claudin-1/CLDN1 and Huh-7.5.1 human hepatoma cells, but not mock-transfected HT1080 cells or non-claudin-1-expressing S7-d6 cells (Courtesy of Professor Masuo Kondoh, PhD, Osaka University, Japan).
      Western Blotting Analysis: 0.5 µg/mL from a representative lot detected exogenously expressed claudin-1 in CLDN1-transfected, but not mock-transfected, HT1080 cells (Courtesy of Professor Masuo Kondoh, PhD, Osaka University, Japan).
      Immunocytochemistry Analysis: A representative lot immunostained the surface of Huh-7.5.1 human hepatoma cells, but not the non-claudin-1-/CLDN1-expressing S7-A cells (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
      Flow Cytometry Analysis: A representative lot specifically immunostained HT1080 cells expressing exogenously transfected human claudin-1 (CLDN1), but not HT1080 cells expressing human claudin-2, -4, -5, -6, -7, or -9, nor L cells expressing mouse claudin-1 (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
      Flow Cytometry Analysis: A representative lot immunostained HEK293T transfectants expressing FLAG-tagged human claudin-1/CLDN1 and human-mouse claudin-1 chimeras with the second human extracellular loop (EL2), but not chimeras with the mouse EL2. M152L, but not V155I, mutation in human EL2 abolished the immunoreactivity (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
      Western Blotting Analysis: A representative lot detected FLAG-tagged human claudin-1/CLDN1 and human-mouse claudin-1 chimeras with the second human extracellular loop (EL2), but not chimeras with the mouse EL2. M152L, but not V155I, mutation in the second human extracellular loop abolished the immunoreactivity (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
      ELISA Analysis: A representative lot detected claudin-1/CLDN1 immunoreactivity in 3.7% formaldehyde-fixed Huh-7.5.1 human hepatoma cells by "cell ELISA" (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
      Neutralization Analysis: A representative lot inhibited HCV infection of cultured Huh-7.5.1 human hepatoma cells in vitro and of human liver-chimeric mice in vivo (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
      Biological Information
      ImmunogenRecombinant human Claudin-1/CLDN1.
      EpitopeSecond extracellular loop.
      Clone7A5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7A5 specifically recognized human claudin-1/CLDN1 by targeting an epitope in the second extracellular loop and prevented claudin-1-dependent hepatitis C virus (HCV) entry into host cells. Clone 7A5 does not react with human claudin-2, -4, -5, -6, -7, or -9, nor mouse claudin-1 (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Not Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CLDN1
      • CLD1
      • ILVASC
      • NISCH
      • SEMP1
      • UNQ481/PRO944
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~20 kDa observed. 22.74 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

      Immunocytochemistry Analysis: 10 µg/mL of this antibody detected Claudin-1/CLDN1 in HepG2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT366 04055977351576

      Documentation

      Anti-Claudin-1/CLDN1 Antibody, clone 7A5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Claudin-1/CLDN1 Antibody, clone 7A5 Certificates of Analysis

      TitleLot Number
      Anti-Claudin-1/CLDN1, clone 7A5 - 3328865 3328865
      Anti-Claudin-1/CLDN1, clone 7A5 - 3784454 3784454
      Anti-Claudin-1/CLDN1, clone 7A5 - 3840850 3840850
      Anti-Claudin-1/CLDN1, clone 7A5 - 3886613 3886613
      Anti-Claudin-1/CLDN1, clone 7A5 - 3928109 3928109
      Anti-Claudin-1/CLDN1, clone 7A5 - 4023282 4023282
      Anti-Claudin-1/CLDN1, clone 7A5 - 4128220 4128220
      Anti-Claudin-1/CLDN1, clone 7A5 - 4206940 4206940
      Anti-Claudin-1/CLDN1, clone 7A5 - 4269974 4269974
      Anti-Claudin-1/CLDN1, clone 7A5 -Q2688172 Q2688172