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AB5592-200UL Anti-GABA A Receptor α1 Antibody

Anti-GABA A Receptor α1 Antibody detects level of GABA A Receptor α1 & has been published & validated for use in IH & WB.

Anti-GABA A Receptor α1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: GABRA1, ECA4, GABA, EJM UniProt Number: P14867
AB5592-200UL
200 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5592-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-GABA A Receptor α1 Antibody
      Alternate Names
      • gamma-aminobutyric acid receptor type A alpha1 subunit
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationAnti-GABA A Receptor α1 Antibody detects level of GABA A Receptor α1 & has been published & validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1.5 μg/mL (1:200) using ECL on rat brain membranes. Recognizes a 51kDa band.

      Immunohistochemistry on rat brain: 4% PFA fixed sections, frozen 1:100-1:200 typical.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPeptide corresponding to amino acids 1-16 from mouse or rat GABA(A) alpha1 subunit (Swiss-Prot accession number P18504, residues 28-43 of the precursor).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes GABA(A) alpha1. The epitope shares little sequence similiarity with alpha5 and alpha2 subunits (respectively, 9/16 and 8/16 residues identical). Does not share homology with any other known proteins.

      SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse. The epitope is highly conserved in human and bovine (16/17) and chicken (14/17). Other species have not been tested.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.
      Gene Symbol
      • GABRA1
      • ECA4
      • GABA
      • EJM
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14867 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
      SIZE: 456 amino acids; 51802 Da
      SUBUNIT: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
      SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
      DISEASE: SwissProt: P14867 # Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME) [MIM:606904]. JME is a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks. & Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. ECA4 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood.
      SIMILARITY: SwissProt: P14867 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB5592-200UL 04053252324413

      Documentation

      Anti-GABA A Receptor α1 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-GABA A Receptor α1 Antibody Certificates of Analysis

      TitleLot Number
      Anti-GABAA Receptor 1 - NG1898228 NG1898228
      Anti-GABAA Receptor α1 - 2430353 2430353
      Anti-GABAA Receptor α1 - 3504403 3504403
      Anti-GABAA Receptor α1 - 3743333 3743333
      Anti-GABAA Receptor α1 -2515121 2515121
      Anti-GABAA Receptor α1 -2676233 2676233
      Anti-GABAA Receptor α1 -2689385 2689385
      RABBIT ANTI-GABA(A) a1 (g-aminobutyric acid receptor type A a1 subunit, GABRA1) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2274248 2274248

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies