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ABN2241 Anti-GLI2

Anti-GLI2, Cat. No. ABN2241, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein GLI2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-GLI2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Gli2, THP UniProt Number: P10070
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ABN2241
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HIH(P), WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN2241
      ReplacesABN506
      DescriptionAnti-GLI2
      Alternate Names
      • Zinc finger protein GLI2
      • GLI family zinc finger protein 2
      • Tax helper protein
      Background InformationZinc finger protein GLI2 (UniProt: P10070; also known as GLI family zinc finger protein 2, Tax helper protein) is encoded by the Gli2 (also known as THP) gene (Gene ID: 2736) in human. Gli2 belongs to the family of Gli C2H2-type zinc-finger proteins and contains 5 C3H2-type zinc fingers. Its N-terminal domain confers transcriptional repressor activity, while the C-terminal domain mediates transcriptional activation. Transcriptional activity of Gli proteins is also regulated through its binding to Suppressor of Fu (Sufu), which is a negative regulator of Hedgehog signaling in Drosophila as well as in vertebrates. Sufu binds to all three Gli proteins with different affinities. Phosphorylation of Gli2 by DYRK2 is shown to block its transcriptional activity and promotes its proteasomal degradation. Gli2 is reported to be essential for normal embryonic development. Gli2 affects ventroposterior mesodermal development through its regulation of Wnt, Brachyury and Xhox3 gene families. Knockout studies have shown that Gli1 can compensate for Gli2, but replacement is not perfect and is context dependent. Gli2 null mice embryos develop neural tube defects and double knockout Gli1 and Gli2 mice display serious central nervous system and lung defects. Overexpression of Gli2 has been linked to tumorigenesis and can lead to basal cell carcinomas. Mutations in Gli2 gene have been linked to holoprosencephaly 9 that is characterized by failure of forebrain to correctly separate into right and left hemispheres. Gli2 gene mutations are also known to cause Culler-Jones syndrome that features pituitary dysfunction, syndactyly, hypothalamic hamartoma, and visceral malformations.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GLI2, Cat. No. ABN2241, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein GLI2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution of this antibody detected GLI2 in human pancreas, human cerebral cortex, and rat testis.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids from the internal edge of N-terminal region of Human Gli2.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects Gli2 in mouse brain. It targets an epitope within 12 amino acids from the N-terminal half of isoform 5.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteMouse, Human.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gli2
      • THP
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~170 kDa observed; 167.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected GLI2 in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN2241 04054839189074