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MABT819 Anti-Glycosaminoglycan Antibody, skin specific Antibody, clone PG-4

Anti-Glycosaminoglycan, skin specific, clone PG-4, Cat. No. MABT819, is a mouse monoclonal antibody that detects glycosaminoglycan and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure
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MABT819
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Shk, ChELISA, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT819
      DescriptionAnti-Glycosaminoglycan Antibody, skin specific Antibody, clone PG-4
      Alternate Names
      • Proteoglycan
      • Dermatan sulfate
      • Chondroitin sulfate
      Background InformationProteoglycans (PG), present in all tissues, are one of the best-studied classes of matrix molecules. Over 50 members of PG have been characterized and studied for their biological significance. PG consist of a protein core to which glycosaminoglycan chains are covalently linked. Glycosaminoglycans (GAGs) are natural heteropolysaccharides that are present in every mammalian tissue. They are composed of repeating disaccharide units that consist of either sulfated or non-sulfated monosaccharides. Their molecular size and the sulfation type vary based on tissue type. The major types of GAGs found in mammalian tissues are: hyaluronan, chondroitin sulfate, and dermatan sulfate, heparin and heparan sulfate, and keratan sulfate. GAG chains are covalently bound to serine residues of the PG protein core via a tetrasaccharide linkage, consisting of xylose, two galactose residues and glucuronic acid. This antibody, clone PG-4, recognizes antigen that is associated with extracellular matrices and surfaces of cultured human dermal fibroblasts and the surfaces of cultured human adult keratinocytes. This antibody recognize native epitopes located within glycosaminoglycan chains and is detects both chondroitin sulfate (Versican) and dermatan sulfate (Decorin and Biglycan) proteoglycans. Treatment of PG with chondroitinases is reported to abolish its immunoreactivity for all antigens. (Ref.: Afratis, N et al. (2012). FEBS Journal 279 (7); 1177-1197; Sorrell, JM et al. (1999). Histochem. J. 31 (8); 549-558).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Glycosaminoglycan, skin specific, clone PG-4, Cat. No. MABT819, is a mouse monoclonal antibody that detects glycosaminoglycan and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Glycosaminoglycan in Western Blotting applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).

      ELISA Analysis: A representative lot detected Glycosaminoglycan in ELISA applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).

      Immunohistochemistry Analysis: A representative lot detected Glycosaminoglycan in Immunohistochemistry applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).
      Biological Information
      ImmunogenCrude membrane fractions from adult human dermal fibroblasts from breast skin.
      ClonePG-4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone PG4 detects glycosaminoglycan in skin cells.
      IsotypeIgMκ
      Species Reactivity
      • Human
      • Shark
      • Chicken
      Species Reactivity NoteHuman, Chicken, Shark.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Molecular Weight~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human skin tissue.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Glycosaminoglycan in human skin tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT819 04054839399787

      Documentation

      Anti-Glycosaminoglycan Antibody, skin specific Antibody, clone PG-4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glycosaminoglycan Antibody, skin specific Antibody, clone PG-4 Certificates of Analysis

      TitleLot Number
      Anti-Glycosaminoglycan, skin specific, clone PG-4 - 3449677 3449677
      Anti-Glycosaminoglycan, skin specific, clone PG-4 - 3770301 3770301
      Anti-Glycosaminoglycan, skin specific, clone PG-4 - 3984021 3984021
      Anti-Glycosaminoglycan, skin specific, clone PG-4 - 4190452 4190452
      Anti-Glycosaminoglycan, skin specific, clone PG-4 - 4278849 4278849
      Anti-Glycosaminoglycan, skin specific, clone PG-4 Monoclonal Antibody Q2954569