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MABF2811-100UL Anti-Hemagglutinin Antibody, Influenza A virus H5N8 Antibody, clone 7H6C

Anti-Hemagglutinin, Influenza A virus H5N8, clone 7H6C, Cat. No. MABF2811, is a mouse monoclonal antibody that detects Hemagglutinin and is used in Affinity Binding Assay, ELISA, Electron Microscopy, Immunofluorescence, Inhibition, and Western Blotting.

MABF2811-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2811-100UL
      DescriptionAnti-Hemagglutinin Antibody, Influenza A virus H5N8 Antibody, clone 7H6C
      Alternate Names
      • HA-H5N8
      Background InformationHemagglutinin (UniProt: A0A096XG14/ A0A0R9BPQ0; also known as HA) is encoded by the HA gene (Gene ID: 3654615) in avian influenza A virus of H5N8 strain. H5N8 strain is a highly pathogenic avian influenza virus. Hemagglutinin (HA), a surface glycoprotein of influenza viruses, is a major target for detection of the influenza virus subtype in the infected samples. HA protrudes from the outside of the virus particles in the form of a trimer. It is a single-pass type I membrane protein with a single transmembrane domain that is localized in amino acids 532-554. HA is cleaved into two chains known as HA1 and HA2 by the action of TMPRSS2 or a furin-like protease of host cells. The cleavage site is localized between amino acids 345 and 346. HA binds to the sialic acid-containing receptors on the cell surface that brings about the attachment of virus to the host cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. It can bind to animal red blood cells and cause a coagulation reaction (hemagglutination). Clone 7H6C binds to HA protein of H5N1 and H5N8, but not to HA proteins of H1N1, H3N2, and H7N9, indicating its specificity for H5-subtypes. (Ref.: Yu-Chen Cheng, Y-C., and Chang, S-C. (2020). Appl. Microbiol. Biotechnol. 105(1); 235-245).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Hemagglutinin, Influenza A virus H5N8, clone 7H6C, Cat. No. MABF2811, is a mouse monoclonal antibody that detects Hemagglutinin and is used in Affinity Binding Assay, ELISA, Electron Microscopy, Immunofluorescence, Inhibition, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Affinity Binding Assay
      • Hemagglutination Inhibition
      • Electron Microscopy
      • ELISA
      Application NotesTested applications

      Inhibition: A representative lot inhibited Hemagglutinin activity in Influenza A virus H5N8 in (Cheng, Y-C. and Chang, SC. (2021). Appl Microbiol Biotechnol. 105(1); 235-245).

      Electron Microscopy: 45 g/mL from a representative lot detected Hemagglutinin, Influenza A virus H5N8 in H5/H6/H7 trivalent VLPs. (Data provided by Dr. Hui-Wen Chen (Department of Veterinary Medicine, National Taiwan University).

      Immunofluorescence Analysis: 4.5 µg/mL from a representative lot detected Hemagglutinin, Influenza A virus H5N8 in Recombinant baculovirus-infected Sf21 cells (Data provided by Dr. Hui-Wen Chen (Department of Veterinary Medicine, National Taiwan University).

      Western Blotting Analysis: A representative lot detected Hemagglutinin, Influenza A virus H5N8 in Western Blotting applications (Cheng, Y-C., and Chang, SC. (2021). Appl Microbiol Biotechnol. 105(1); 235-245).

      Affinity Binding Assay: A representative lot detected Hemagglutinin, Influenza A virus H5N8 in Affinity Binding applications (Cheng, Y-C., and Chang, SC. (2021). Appl Microbiol Biotechnol. 105(1;235-245).

      ELISA Analysis: A representative lot detected Hemagglutinin, Influenza A virus H5N8 in ELISA applications (Cheng, Y-C., and Chang, SC. (2021). Appl Microbiol Biotechnol. 105(1); 235-245).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged recombinant HA1 or HA2 proteins of Avian Influenza virus, H5N8 strain, expressed in E.coli.
      EpitopeN-terminal half
      Clone7H6C
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 7H6C is a mouse monoclonal antibody that detects hemagglutinin of Avian Influenza virus, H5N8 strain. It targets an epitope within 24 amino acids from the N-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteInfluenza A Virus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • HA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~60 kDa observed; 64.1 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with full-length, recombinant Hemagglutinin HA from the H5N8 strain of Avian influenza A virus.

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected full-length, recombinant Hemagglutinin HA from the H5N8 strain of Avian influenza A virus, but not the recombinant Hemagglutinin HA2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2811-100UL 04065269802327