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ABS2129 Anti-IP3R 1

Detect Inositol 1,4,5-trisphosphate receptor type 1 using this rabbit polyclonal Anti-IP3R1, Cat. No. ABS2129 that has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. ,

Anti-IP3R 1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Itpr1, Insp3r UniProt Number: P29994
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ABS2129
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, Ch, B, HIP, WB, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS2129
      DescriptionAnti-IP3R 1
      Alternate Names
      • Inositol 1
      • 4
      • 5-trisphosphate receptor type I
      • IP3 receptor isoform 1
      • IP-3-R1
      • InsP3R1
      • Type 1 inositol 1
      • 4
      • 5-trisphosphate receptor
      • Type 1 InsP3 receptor
      Background InformationInositol 1,4,5-trisphosphate receptor type 1 (UniProt: P29994; also known as IP3 receptor isoform 1, IP-3-R, IP3R 1, InsP3R1, Type 1 inositol 1,4,5-trisphosphate receptor, Type 1 InsP3 receptor) is encoded by the Itpr1 (also known as Insp3r) gene (Gene ID: 25262) in rat. InsP3R1 is a multi-pass membrane protein located on endoplasmic reticulum (ER) membrane. Its highest levels are found in neurons, with very high expression in the Purkinje cells of the cerebellum. InsP3R1 contains a calcium channel in its C-terminal extremity. It has a large N-terminal cytoplasmic region that has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. InsP3R1 serves as an intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.It is involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1. It also plays a role in ER stress-induced apoptosis wherein cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways. InsP3R1 is phosphorylated by cAMP-dependent protein kinase (PKA), which increases the interaction with inositol 1,4,5-trisphosphate and reduces the interaction with AHCYL1. This prevents the ligand-induced opening of the calcium channels. Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium-binding protein which then inhibits the receptor. Reduced expression of InsP3R1 has been reported in cisplatin-resistant cancer cells and overexpression of InsP3R1 in resistant cells induces apoptosis and increases sensitivity to cisplatin. (Ref.: Tsunoda T., et al. (2005). Oncogene. 24(8):1396-402; Wang, Y., et al. (2001). Cir. Res. 88(2): 202-209).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 1% BSA and 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Inositol 1,4,5-trisphosphate receptor type 1 using this rabbit polyclonal Anti-IP3R1, Cat. No. ABS2129 that has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. ,
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected IP3R1 in 10 µg of rat and mouse brain microsomal preparation.

      Western Blotting Analysis: A representative lot detected IP3R1 in bovine salivary gland, DT40 3KO cells stably expressing IP3R1 (Chandrasekhar, R., et. al. (2016). J Biol Chem. 291(10):4846-60).

      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected IP3R1 in human brain and rat brain tissues.

      Immunoprecipitation Analysis: A representative lot detected IP3R1 in bovine salivary gland (Chandrasekhar, R., et. al. (2016). J Biol Chem. 291(10):4846-60).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of rat Inositol 1,4,5-trisphosphate receptor type 1 (IP3R1).
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody specifically react with IP3 Receptor 1 in multiple species, but does not recognize IP3R2 and IP3R3. It targets an epitope within 19 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      • Chicken
      • Bovine
      • Human
      Species Reactivity NoteBovine, Chicken, Human, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Itpr1
      • Insp3r
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~300 kDa observed; 313.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain microsomal preparation.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IP3R1 in 10 µg of rat brain microsomal preparation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2129 04054839197710