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ABE499 Anti-JMJD2D Antibody

Detect Jumonji domain-containing protein (JMJD) using this rabbit polyclonal antibody, Anti-JMJD2D Antibody validated for use in western blotting, IHC & IP.

Anti-JMJD2D Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: KDM4D, JHDM3D, JMJD2D UniProt Number: Q6B0I6
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ABE499
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, ChpWB, IHC, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE499
      DescriptionAnti-JMJD2D Antibody
      Alternate Names
      • Lysine-specific demethylase 4D
      • JmjC domain-containing histone demethylation protein 3D
      • Jumonji domain-containing protein 2D
      Background InformationJumonji domain-containing protein 2D (JMJD2D) is also called Lysine-specific demethylase 4D and JmjC domain-containing histone demethylation protein 3D. JMJD2D is a histone demethylase that specifically demethylates both di- and trimethylated H3 'Lys-9', but not monomethylated histone H3 'Lys-9' residues. JMJD2D also does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' or H4 'Lys-20'.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Jumonji domain-containing protein (JMJD) using this rabbit polyclonal antibody, Anti-JMJD2D Antibody validated for use in western blotting, IHC & IP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot detected JMJD2D in human testis and liver tissues.

      Immunoprecipitation Analyis: 20 µg from a representative lot detected JMJD2D in 500 µg of HeLa cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human JMJD2D.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Chimpanzee
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Chimpanzee based on 100% sequence homology. Other homologies: Bovine, Porcine, Canine, and Rhesus Macaque (93% sequence homology), Mouse (87% sequence homology), Rat (80% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KDM4D
      • JHDM3D
      • JMJD2D
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~58 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected JMJD2D in 10 µg of HeLa cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE499 04053252912672