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MABS1250 Anti-Mineralocorticoid Receptor Antibody, clone 2D6

Detect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 2D6 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: Nr3c2, Mlr UniProt Number: P22199
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MABS1250
200 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWB, IHC, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1250
      DescriptionAnti-Mineralocorticoid Receptor Antibody, clone 2D6
      Alternate Names
      • MR
      • Nuclear receptor subfamily 3 group C member 2
      • Mineralocorticoid Receptor
      Background InformationMineralocorticoid receptor (UniProt P22199; also known as MR, Nuclear receptor subfamily 3 group C member 2) is encoded by the Nr3c2 (also known as MCR, Mlr) gene (Gene ID 25672) in rats. Mineralocorticoid receptor (MR) is expressed in multiple tissues, including renal epithelia, smooth muscle, endothelium, cardiomyocytes, and hippocampal neurons, where it mediates diverse functions. MR is activated by aldosterone and also by cortisol in cells that do not express 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2). MR is normally activated by aldosterone, which is produced by adrenal glomerulosa in response to intravascular volume depletion and hyperkalemia. Consistently, gain of function mutations in MR lead to severe hypertension, often with hypokalemia, while loss of function mutations result in neonatal hypotension. Ser843 phosphorylation in the MR ligand-binding domain is reported to prevent MR ligand binding and activation. MR pS843 is found exclusively in intercalated cells of the distal nephrons in the kidney. Intravascular volume depletion, angiotensin II and WNK4 signaling reduce MR pS843 levels, whereas hyperkalemia increases MR pS843.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 2D6 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in hippocampus, dentate gyrate, choroid plexus, heart, and rat kidney (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
      Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
      Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in rat hippocampal cytosolic preparation and exogenously expressed EGFP-rat MR fusion protein in CHO cells (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
      Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney cytosolic fraction and exogenously expressed human MR in COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671)
      Biological Information
      ImmunogenConjugated linear peptide corresponding to the N-terminus of rat Mineralocorticoid Receptor.
      EpitopeNear N-terminus
      Clone2D6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityEpitope is present in all 3 alternative spliced isoforms
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nr3c2
      • Mlr
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~107 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain cytosol tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of rat brain cytosol tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1250 04055977173031

      Documentation

      Anti-Mineralocorticoid Receptor Antibody, clone 2D6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Mineralocorticoid Receptor Antibody, clone 2D6 Certificates of Analysis

      TitleLot Number
      Anti-Mineralocorticoid Receptor, -Q2573211 Q2573211
      Anti-Mineralocorticoid Receptor, clone 2D6 - 3245604 3245604
      Anti-Mineralocorticoid Receptor, clone 2D6 - 3884760 3884760
      Anti-Mineralocorticoid Receptor, clone 2D6 - 4209927 4209927

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies