AB3704 Anti-Orexin-A Antibody

AB3704
50 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIHCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB3704
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Orexin-A Antibody
      References
      Product Information
      FormatSerum
      PresentationRabbit serum. Lyophilized. Reconstitute with 50 mL of sterile distilled water. Centrifuge to remove any insoluble material. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationDetect Orexin-A using this Anti-Orexin-A Antibody validated for use in IH.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:1,000-1:2,000. The antibody shows intense labeling of Orexin-A neurons with low background.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the C-terminal portion of the bovine Orexin-A peptide.
      HostRabbit
      SpecificityOrexin-A.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a hypothalamic neuropeptide precusor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.
      Gene Symbol
      • HCRT
      • Hypocretin
      • OX
      • orexin
      • Hcrt
      • PPOX
      • PPORX
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43612 # Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
      SIZE: 131 amino acids; 13363 Da
      SUBCELLULAR LOCATION: Rough endoplasmic reticulum (By similarity). Cytoplasmic vesicle (By similarity). Cell junction, synapse (By similarity). Note=Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses (By similarity).
      TISSUE SPECIFICITY: Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
      PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides.
      DISEASE: SwissProt: O43612 # Defects in HCRT are a cause of narcolepsy [MIM:161400]. Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed.
      SIMILARITY: SwissProt: O43612 ## Belongs to the orexin family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material frozen at -20°C to -70°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1, ACS or better grade) can be added for additional stability.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Orexin-A Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Orexin-A Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-OREXIN-A - 28348092834809
      RABBIT ANTI-OREXIN-A - 31725763172576
      RABBIT ANTI-OREXIN-A - 31767503176750
      RABBIT ANTI-OREXIN-A - 31782583178258
      RABBIT ANTI-OREXIN-A - 31782593178259
      RABBIT ANTI-OREXIN-A - 31782603178260
      RABBIT ANTI-OREXIN-A - 31886803188680
      RABBIT ANTI-OREXIN-A - 32048973204897
      RABBIT ANTI-OREXIN-A - 32139853213985
      RABBIT ANTI-OREXIN-A - 32515013251501

      References

      Reference overviewApplicationSpeciesPub Med ID
      Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish.
      Singh, C; Oikonomou, G; Prober, DA
      eLife  4  e07000  2015

      Show Abstract
      26374985 26374985
      Leptin receptor neurons in the mouse hypothalamus are colocalized with the neuropeptide galanin and mediate anorexigenic leptin action.
      Laque, A; Zhang, Y; Gettys, S; Nguyen, TA; Bui, K; Morrison, CD; Münzberg, H
      American journal of physiology. Endocrinology and metabolism  304  E999-1011  2013

      Show Abstract
      ImmunohistochemistryMouse23482448 23482448
      Neuropeptide S promotes wakefulness through activation of the posterior hypothalamic histaminergic and orexinergic neurons.
      P Zhao,Y F Shao,M Zhang,K Fan,X P Kong,R Wang,Y P Hou
      Neuroscience  207  2012

      Show Abstract
      22300983 22300983
      Organization and number of orexinergic neurons in the hypothalamus of two species of Cetartiodactyla: a comparison of giraffe (Giraffa camelopardalis) and harbour porpoise (Phocoena phocoena).
      Dell, LA; Patzke, N; Bhagwandin, A; Bux, F; Fuxe, K; Barber, G; Siegel, JM; Manger, PR
      Journal of chemical neuroanatomy  44  98-109  2012

      Show Abstract
      22683547 22683547
      Orexins in the midline thalamus are involved in the expression of conditioned place aversion to morphine withdrawal.
      Yonghui Li,Huiying Wang,Keke Qi,Xiaoyu Chen,Sa Li,Nan Sui,Gilbert J Kirouac
      Physiology & behavior  102  2011

      Show Abstract
      20951152 20951152
      Distribution of orexinergic neurons and their terminal networks in the brains of two species of African mole rats.
      Adhil Bhagwandin,Kjell Fuxe,Nigel C Bennett,Paul R Manger
      Journal of chemical neuroanatomy  41  2011

      Show Abstract
      21093582 21093582
      Differential distribution of melanin-concentrating hormone (MCH)- and hypocretin (Hcrt)-immunoreactive neurons projecting to the mesopontine cholinergic complex in the rat.
      Eun Y Hong,Ye S Yoon,Hyun S Lee
      Brain research  1424  2011

      Show Abstract
      22015351 22015351
      Cellular location and major terminal networks of the orexinergic system in the brains of five microchiropteran species.
      Kruger, Jean-Leigh, et al.
      J. Chem. Neuroanat., 40: 256-62 (2010)  2010

      Show Abstract
      20654711 20654711
      Projections from the paraventricular nucleus of the thalamus to the forebrain, with special emphasis on the extended amygdala.
      Sa Li,Gilbert J Kirouac
      The Journal of comparative neurology  506  2008

      Show Abstract
      18022956 18022956
      How does immune challenge inhibit ingestion of palatable food? Evidence that systemic lipopolysaccharide treatment modulates key nodal points of feeding neurocircuitry.
      Park, SM; Gaykema, RP; Goehler, LE
      Brain, behavior, and immunity  22  1160-72  2008

      Show Abstract Full Text Article
      18562160 18562160

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies